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Metabolome and fecal microbiota in monozygotic twin pairs discordant for weight: a Big Mac challenge
VTT Technical Research Centre of Finland, Espoo, Finland; Department of Food Science and Physiology, University of Navarra, Pamplona, Spain.
VTT Technical Research Centre of Finland, Espoo, Finland.
VTT Technical Research Centre of Finland, Espoo, Finland.
Obesity Research Unit, Research Programs Unit, Diabetes and Obesity, University of Helsinki, Helsinki, Finland; Department of Psychiatry, Helsinki University Central Hospital, Helsinki, Finland.
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2014 (English)In: The FASEB Journal, ISSN 0892-6638, E-ISSN 1530-6860, Vol. 28, no 9, p. 4169-4179Article in journal (Refereed) Published
Abstract [en]

Postprandial responses to food are complex, involving both genetic and environmental factors. We studied postprandial responses to a Big Mac meal challenge in monozygotic co-twins highly discordant for body weight. This unique design allows assessment of the contribution of obesity, independent of genetic liability. Comprehensive metabolic profiling using 3 analytical platforms was applied to fasting and postprandial serum samples from 16 healthy monozygotic twin pairs discordant for weight (body mass index difference >3 kg/m(2)). Nine concordant monozygotic pairs were examined as control pairs. Fecal samples were analyzed to assess diversity of the major bacterial groups by using 5 different validated bacterial group specific denaturing gradient gel electrophoresis methods. No differences in fecal bacterial diversity were detected when comparing co-twins discordant for weight (ANOVA, P<0.05). We found that within-pair similarity is a dominant factor in the metabolic postprandial response, independent of acquired obesity. Branched chain amino acids were increased in heavier as compared with leaner co-twins in the fasting state, but their levels converged postprandially (paired t tests, FDR q<0.05). We also found that specific bacterial groups were associated with postprandial changes of specific metabolites. Our findings underline important roles of genetic and early life factors in the regulation of postprandial metabolite levels.

Place, publisher, year, edition, pages
2014. Vol. 28, no 9, p. 4169-4179
Keywords [en]
bile acids, metabolomics, obesity
National Category
Nutrition and Dietetics Endocrinology and Diabetes
Identifiers
URN: urn:nbn:se:oru:diva-63695DOI: 10.1096/fj.14-250167ISI: 000340845900033PubMedID: 24846387Scopus ID: 2-s2.0-84907202489OAI: oai:DiVA.org:oru-63695DiVA, id: diva2:1169260
Funder
Novo Nordisk
Note

Funding agencies:

EU 222720 277713

Academy of Finland Centre of Excellence in Molecular Systems Immunology and Physiology Research (SyMMyS) 250114

Finnish Foundation for Cardiovascular Research

Diabetes Research Foundation

Helsinki University Central Hospital Research Funds

Academy of Finland grant 266286

Carlos III Health Institute of the Spanish Ministry of Health

Available from: 2017-12-22 Created: 2017-12-22 Last updated: 2018-01-30Bibliographically approved

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Hyötyläinen, TuuliaOresic, Matej

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