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Quantitative profiling of bile acids in blood, adipose tissue, intestine, and gall bladder samples using ultra high performance liquid chromatography-tandem mass spectrometry
VTT Technical Research Center of Finland, Espoo, Finland; Finnish Food Safety Authority Evira, Helsinki, Finland.
VTT Technical Research Center of Finland, Espoo, Finland.
VTT Technical Research Center of Finland, Espoo, Finland.
Obesity Research Unit, Research Programs Unit, Diabetes and Obesity, University of Helsinki, Helsinki, Finland; Department of Medicine, Division of Endocrinology, Helsinki University Central Hospital, Helsinki, Finland; FIMM, Institute for Molecular Medicine University of Helsinki, Helsinki, Finland.
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2014 (English)In: Analytical and Bioanalytical Chemistry, ISSN 1618-2642, E-ISSN 1618-2650, Vol. 406, no 30, p. 7799-7815Article in journal (Refereed) Published
Abstract [en]

An ultra high performance liquid chromatography tandem mass spectrometry method (UHPLC-MS/MS) was developed for the determination of 33 target and 28 unknown bile acids (BAs) in biological samples. Sixty-one BAs could be measured in 20 min using only a small amount of sample and with a simple sample preparation. The method proved to be very sensitive (limit of detection 5-350 pg/mL, lower limit of quantitation 0.1-2.6 ng/mL), linear (R(2) > 0.99) and reproducible (typically CV <15 % in biological matrixes). The method was used to analyze human adipose tissue, plasma, and serum (from same subjects) and mouse serum, gall bladder, small intestine, and colon samples (from same animals). Cholic acid, ursodeoxycholic acid, and chenodeoxycholic acid, deoxycholic acid, and their conjugates (mainly glycine, but also taurine conjugates) were the main metabolites in human samples, and cholic acid, glycine cholic acid, and several taurine conjugates in mouse samples. Using the method, 28 unknown BAs could also be detected. UHPLC-MS/MS spectra, accurate mass, and tissue distribution suggested that nine of the unknown bile acids were taurine conjugates, 13 were glycine conjugates, and six were intact BAs, respectively. To our knowledge, this was the first time BAs were detected in adipose tissue. Results showed that 17 targeted BAs were found at ng/g level in human adipose tissue. Our findings give a novel insight of the endogenous role of BAs in adipose tissue and their role as biomarkers (e.g., in metabolic diseases).

Place, publisher, year, edition, pages
Springer, 2014. Vol. 406, no 30, p. 7799-7815
National Category
Analytical Chemistry Endocrinology and Diabetes
Identifiers
URN: urn:nbn:se:oru:diva-63698DOI: 10.1007/s00216-014-8230-9ISI: 000345377600004PubMedID: 25384335Scopus ID: 2-s2.0-84914096140OAI: oai:DiVA.org:oru-63698DiVA, id: diva2:1169263
Funder
EU, FP7, Seventh Framework Programme, FP7-KBBE-222720
Note

Funding agencies:

Academy of Finland Centre of Excellence in Molecular Systems in Immunology and Physiology research SyMMys 250114 

Available from: 2017-12-22 Created: 2017-12-22 Last updated: 2018-01-30Bibliographically approved

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Oresic, MatejHyötyläinen, Tuulia

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