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Testosterone-like immunoreactivity in hair measured in minute sample amounts - a competitive radioimmunoassay with an adequate limit of detection
Department of Clinical and Experimental Medicine, Linköping University, Linköping, Sweden; Department of Clinical Chemistry, Linköping University, Linköping, Sweden.
Örebro University, School of Medical Sciences. Örebro University Hospital. Department of Neurology, Örebro University Hospital, Örebro, Sweden.ORCID iD: 0000-0002-6681-0546
Department of Clinical and Experimental Medicine, Linköping University, Linköping, Sweden; Department of Clinical Chemistry, Linköping University, Linköping, Sweden.
2017 (English)In: Scientific Reports, E-ISSN 2045-2322, Vol. 7, article id 17636Article in journal (Refereed) Published
Abstract [en]

The concentrations of testosterone deposited in hair during hair growth may provide a retrospective reflection of the concentrations of bioactive testosterone in plasma. The objective of this study was to develop a radioimmunoassay with a sufficiently low limit of detection to measure the testosterone-like immunoreactivity in smaller hair samples (5 mg) than used in earlier studies, and to compare three different extraction procedures. The competitive radioimmunoassay consisted of a polyclonal antiserum (immunogen testosterone-7 alpha-BSA) and a radioligand synthesised from testosterone-3-CMO-histamine. The within-assay and total coefficients of variation in the working range was 3% and 4.5%, respectively. The limit of detection was 0.87 pg/mL, which is equivalent to 0.12 pg/mg testosterone in 5 mg of hair. The concentration of testosterone-like immunoreactivity in hair samples was 1.23 (SD 0.47) pg/mg in women and 2.67 (SD 0.58) pg/mg in men (pulverised hair). Significantly improved precision was found when pulverised hair was used compared to non-pulverised hair. Our data indicate that pulverisation of the hair prior to hormone extraction is crucial. Detection limits fit for the intended purpose are achievable with 5 mg samples of hair.

Place, publisher, year, edition, pages
Nature Publishing Group, 2017. Vol. 7, article id 17636
National Category
Analytical Chemistry Occupational Health and Environmental Health
Identifiers
URN: urn:nbn:se:oru:diva-63817DOI: 10.1038/s41598-017-17930-wISI: 000418250800021PubMedID: 29247184Scopus ID: 2-s2.0-85038244979OAI: oai:DiVA.org:oru-63817DiVA, id: diva2:1170632
Note

Funding Agency:

County Council of Östergötland

Available from: 2018-01-04 Created: 2018-01-04 Last updated: 2022-09-15Bibliographically approved

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Ström, Jakob O.

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