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Exerciseand insulin cause GLUT-4 translocation in human skeletal muscle
Department of Surgery, Huddinge University Hospital, Huddinge, Sweden.
Department of Medicine, Brigham and Women's Hospital, Harvard Medical School, Boston MA, United States.
Department of Surgery, Karolinska Hospital, Karolinska Institute, Stockholm, Sweden.
Department of Thorac. Clinical Physiology, Karolinska Hospital, Karolinska Institute, Stockholm, Sweden.
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1999 (English)In: American Journal of Physiology, ISSN 0002-9513, E-ISSN 2163-5773, Vol. 277, no 4, p. E733-E741Article in journal (Refereed) Published
Abstract [en]

Studies in rodents have established that GLUT-4 translocation is the major mechanism by which insulin and exercise increase glucose uptake in skeletal muscle. In contrast, much less is known about the translocationphenomenon in human skeletal muscle. In the current study, nine healthy volunteers were studied on two different days. On one day, biopsies of vastus lateralis muscle were taken before and after a 2-h euglycemic- hyperinsulinemic clamp (0.8 mU · kg-1 · min-1). On another day, subjects exercised for 60 min at 70% of maximal oxygen consumption (VO(2max)), a biopsy was obtained, and the same clamp and biopsy procedure was performed as that during the previous experiment. Compared with insulin treatment alone, glucose infusion rates were significantly increased during the postexercise clamp for the periods 0-30 min, 30-60 min, and 60-90 min, but not during the last 30 min of the clamp. Plasma membrane GLUT-4 content was significantly increased in response to physiological hyperinsulinemia (32% above rest), exercise (35%), and the combination of exercise plus insulin(44%). Phosphorylation of Akt, a putative signaling intermediary for GLUT-4 translocation, was increased inresponse to insulin (640% above rest), exercise (280%), and exercise plus insulin (1,000%). These data demonstrate that two normal physiological conditions, moderate intensity exercise and physiological hyperinsulinemia ~56 μU/ml, cause GLUT-4 translocation and Akt phosphorylation in human skeletal muscle.

Place, publisher, year, edition, pages
American Physiological Society , 1999. Vol. 277, no 4, p. E733-E741
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Physiology
Identifiers
URN: urn:nbn:se:oru:diva-63897ISI: 000083919000020PubMedID: 10516134Scopus ID: 2-s2.0-0032758457OAI: oai:DiVA.org:oru-63897DiVA, id: diva2:1171096
Note

Funding agencies:

NIAMS NIH HHS: AR-45670 ,AR-42238 

Available from: 2018-01-05 Created: 2018-01-05 Last updated: 2018-02-05Bibliographically approved

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