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Vitamin D C3 epimer in a mid-Swedish region: Analytical measurement and epidemiology
Örebro University, School of Medical Sciences. Department of Endocrinology.
Department of Clinical Chemistry, Örebro University Hospital, Örebro, Sweden.
Örebro University, School of Medical Sciences. Department of Pediatrics.ORCID iD: 0000-0002-1440-9961
Department of Endocrinology, School of Medical Sciences, Örebro University, Örebro, Sweden.
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2018 (English)In: Clinica Chimica Acta, ISSN 0009-8981, E-ISSN 1873-3492, Vol. 3, no 478, 182-187 p.Article in journal (Refereed) Epub ahead of print
Abstract [en]

Background: The discovery of an epimeric form of 25(OH)D3 may complicate the interpretation of vitamin Dstatus. The aim of this study was to examine the prevalence and determinants of 25-hydroxy-3-epi-vitamin D3 (3- epi-25(OH)D3) in a mid-Swedish region and to investigate how the measurement of 3-epi-25(OH)D3 would affect the assessment of vitamin D status using current thresholds.

Methods: We conducted a cross-sectional study of 8286 in- and outpatients in primary as well as secondary care settings. Plasma 25(OH)D, 25(OH)D2, 25(OH)D3 and 3-epi-25(OH)D3 were measured using High Pressure Liquid Chromatography – Tandem Mass Spectrometry (HPLC – MS/MS). The relative 3-epi-25(OH)D3 contribution was calculated as a percentage of the total 25(OH)D3. Blood samples were collected between March 2014 and July 2015 providing a seasonal aspect to the results.

Results: 3-epi-25(OH)D3was detected in 635 cases (7.7% of all subjects), and the mean concentration was8.4 ± 3.5 nmol/L. 3-epi-25(OH)D3correlated significantly with 25(OH)D3(r =0.38, p < 0.001).A multivariateanalysis among the detected showed that male gender and winter season were independently associatedwith higher 3-epi-25(OH)D3/25(OH)D3percentage ratio (R2=0.044). Infants and children had a significantlyhigher detection rate compared to the reference age category (18–45 years) as well as those who were testedduring the summer season.

Conclusions: We report findings from the first epidemiologic study of 3-epi-25(OH)D3 conducted in Sweden, based on a large population sample. 3-epi-25(OH)D3 was detected in 7.7% of the study population and the mean concentration was 8.4 nmol/L. The quantification of 3-epi-25(OH)D3 would not significantly influence the clinical interpretation of vitamin D levels. Additional studies are needed to understand the metabolic pathway and the possible physiological functions of this metabolite.

Place, publisher, year, edition, pages
Amsterdam, Netherlands: Elsevier, 2018. Vol. 3, no 478, 182-187 p.
Keyword [en]
3-epi 25(OH)D(3), 25(OH)D(3), High Pressure Liquid Chromatography – Tandem Mass Spectrometry (HPLC – MS/MS), Vitamin D
National Category
Medical and Health Sciences
Research subject
Medicine
Identifiers
URN: urn:nbn:se:oru:diva-64072DOI: 10.1016/j.cca.2018.01.002PubMedID: 29305842OAI: oai:DiVA.org:oru-64072DiVA: diva2:1173314
Available from: 2018-01-12 Created: 2018-01-12 Last updated: 2018-01-12Bibliographically approved

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Karefylakis, ChristosSärnblad, Stefan

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