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AMP-activated protein kinase (AMPK) is activated in muscle of subjects with type 2 diabetes during exercise
Research Division, Joslin Diabetes Center, Brigham and Women's Hospital, Boston, MA, United States.
Research Division, Joslin Diabetes Center, Brigham and Women's Hospital, Boston, MA, United States.
Research Division, Joslin Diabetes Center, Brigham and Women's Hospital, Boston, MA, United States.
Karolinska Institute, Centre of Gastrointestinal Disease, Ersta Hospital, Stockholm, Sweden.
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2001 (English)In: Diabetes, ISSN 0012-1797, E-ISSN 1939-327X, Vol. 50, no 5, p. 921-927Article in journal (Refereed) Published
Abstract [en]

Insulin-stimulated GLUT4 translocation is impaired in people with type 2 diabetes. In contrast, exercise results in a normal increase in GLUT4 translocation and glucose uptake in these patients. Several groups have recently hypothesized that exercise increases glucose uptake via an insulin-independent mechanism mediated by the activation of AMP-activated protein kinase (AMPK). If this hypothesis is correct, people with type 2 diabetes should have normal AMPK activation in response to exercise. Seven subjects with type 2 diabetes and eight matched control subjects exercised on a cycle ergometer for 45 min at 70% of maximum workload. Biopsies of vastus lateralis muscle were taken before exercise, after 20 and 45 min of exercise, and at 30 min postexercise. Blood glucose concentrations decreased from 7.6 to 4.77 mmol/l with 45 min of exercise in the diabetic group and did not change in the control group. Exercise significantly increased AMPK α2 activity 2.7-fold over basal at 20 min in both groups and remained elevated throughout the protocol, but there was no effect of exercise on AMPK α1 activity. Subjects with type 2 diabetes had similar protein expression of AMPK α1, α2, and β1 in muscle compared with control subjects. AMPK α2 was shown to represent approximately two-thirds of the total a mRNA in the muscle from both groups. In conclusion, people with type 2 diabetes have normal exercise-induced AMPK α2 activity and normal expression of the α1, α2 and β1 isoforms. Pharmacological activation of AMPK may be an attractive target for the treatment of type 2 diabetes.

Place, publisher, year, edition, pages
Alexandria, VA, USA: American Diabetes Association Inc. , 2001. Vol. 50, no 5, p. 921-927
National Category
Medical and Health Sciences Endocrinology and Diabetes
Identifiers
URN: urn:nbn:se:oru:diva-64074DOI: 10.2337/diabetes.50.5.921ISI: 000168392000002PubMedID: 11334434Scopus ID: 2-s2.0-0035039274OAI: oai:DiVA.org:oru-64074DiVA, id: diva2:1173346
Note

Funding Agency:

NIAMS NIH HHS

Available from: 2018-01-12 Created: 2018-01-12 Last updated: 2018-01-16Bibliographically approved

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