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A quantitative PCR measurement of messenger RNA expression of IGF-I, IGF-II and IGFBP-5 in human skeletal muscle
Endocrine Research Unit, Division of Endocrinology and Metabolism, Mayo Clinic and Mayo Foundation, Rochester, MN, USA.
Department of Medicine, University of Vermont, Burlington, VT, USA.ORCID iD: 0000-0003-2636-4745
Department of Medicine, University of Vermont, Burlington, VT, USA.
Endocrine Research Unit, Division of Endocrinology and Metabolism, Mayo Clinic and Mayo Foundation, Rochester, MN, USA.
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1999 (English)In: Growth Hormone & IGF Research, ISSN 1096-6374, E-ISSN 1532-2238, Vol. 9, no 3, p. 179-186Article in journal (Refereed) Published
Abstract [en]

Insulin-like growth factor-I and -II (IGF-I and IGF-II) and their binding proteins are important components in growth promotion and tissue maintenance. We determined the presence of IGF-I, -II, and binding protein 5 (IGFBP-5) gene expression in human skeletal muscle and that mRNA abundance is not altered by nutrients and insulin. In the first protocol, (control) subjects were given water. In the second protocol, half of these subjects drank Polycose (carbohydrate) and the remaining subjects drank equal calories as a mixed meal. Quadriceps muscle biopsies were taken at 10 h. A semi-quantitative polymerase chain reaction was designed to measure gene expression. IGF-I, IGF-II and IGFBP-5 mRNA are present in adult human skeletal muscle, but no significant changes between meal groups were observed for IGF-I, IGF-II or IGFBP-5 mRNA levels, indicating that the expression of these genes are not altered acutely by nutrients and insulin.

Place, publisher, year, edition, pages
Elsevier, 1999. Vol. 9, no 3, p. 179-186
Keywords [en]
QPCR, mRNA, insulin-like growth factor, binding proteins
National Category
Medical and Health Sciences Endocrinology and Diabetes Cell Biology
Identifiers
URN: urn:nbn:se:oru:diva-64370DOI: 10.1054/ghir.1999.0104ISI: 000082872300004PubMedID: 10502454Scopus ID: 2-s2.0-0032836117OAI: oai:DiVA.org:oru-64370DiVA, id: diva2:1175122
Available from: 2018-01-17 Created: 2018-01-17 Last updated: 2018-01-19Bibliographically approved

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Ljungqvist, Olle

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