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Detection of Lysosomal Exocytosis in Platelets by Flow Cytometry
Clinical Chemistry, Department of Clinical and Experimental Medicine, Linköping University, Linköping, Sweden.
Örebro University, School of Medical Sciences. Department of Clinical Medicine, Faculty of Health and Medical Sciences, Örebro University, Örebro, Sweden; Clinical and Experimental Medicine, Linköping University, Linköping, Sweden; Department of Clinical Chemistry, Linköping University, Linköping, Sweden.
2017 (English)In: Methods in Molecular Biology, ISSN 1064-3745, E-ISSN 1940-6029, Vol. 1594, p. 191-203Article in journal (Refereed) Published
Abstract [en]

Flow cytometry is a method that allows high throughput analysis of individual cells in suspension. By inclusion of fluorescently labelled antibodies, it is possible to analyze the abundance of one or more surface antigens, such as LAMP-1, without prior lysis of cells. Here we describe the special considerations required for the investigation of lysosomal exocytosis from platelets analyzed with flow cytometry.

Place, publisher, year, edition, pages
Springer, 2017. Vol. 1594, p. 191-203
Keywords [en]
Flow cytometry, Granule, Lysosomal exocytosis, Lysosomal-associated membrane protein-1 (LAMP-1), Lysosome, Platelet, Platelet activation, Platelet secretion
National Category
Medical and Health Sciences Immunology
Identifiers
URN: urn:nbn:se:oru:diva-64428DOI: 10.1007/978-1-4939-6934-0_12PubMedID: 28456984Scopus ID: 2-s2.0-85018271844OAI: oai:DiVA.org:oru-64428DiVA, id: diva2:1176000
Available from: 2018-01-19 Created: 2018-01-19 Last updated: 2018-08-13Bibliographically approved

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