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Molecular engineering of a thermostable carbohydrate-binding module
Department of Immunotechnology, Lund University, Lund.
Department of Biotechnology, Lund University, Lund.
Alligator Bioscience, Lund, Sweden.
Department of Biotechnology, Lund University, Lund.
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2006 (English)In: Biocatalysis and Biotransformation, ISSN 1024-2422, E-ISSN 1029-2446, Vol. 24, no 1-2, p. 31-37Article in journal (Refereed) Published
Abstract [en]

Structure-function studies are frequently practiced on the very diverse group of natural carbohydrate-binding modules in order to understand the target recognition of these proteins. We have taken a step further in the study of carbohydrate-binding modules and created variants with novel binding properties by molecular engineering of one such molecule of known 3D-structure. A combinatorial library was created from the sequence encoding a thermostable carbohydrate-binding module, CBM4-2 from a Rhodothermus marinus xylanase, and phage-display technology was successfully used for selection of variants with specificity towards different carbohydrate polymers (birchwood xylan, Avicel (TM), ivory nut mannan and recently also xyloglucan), as well as towards a glycoprotein (human IgG4). Our work not only generated a number of binders with properties that would suite a range of biotechnological applications, but analysis of selected binders also helped us to identify residues important for their specificities.

Place, publisher, year, edition, pages
Informa Healthcare, 2006. Vol. 24, no 1-2, p. 31-37
Keywords [en]
binding specificity; carbohydrate-binding module; combinatorial library; molecular engineering; phage-display; protein scaffold
National Category
Biochemistry and Molecular Biology
Identifiers
URN: urn:nbn:se:oru:diva-64490DOI: 10.1080/10242420500518516ISI: 000239089700005Scopus ID: 2-s2.0-33646838036OAI: oai:DiVA.org:oru-64490DiVA, id: diva2:1177114
Conference
6th Carbohydrate Bioengineering Meeting (CBM6), Barcelona, Spain, April 3-6, 2005
Funder
Swedish Research CouncilAvailable from: 2018-01-24 Created: 2018-01-24 Last updated: 2018-02-20Bibliographically approved

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Gunnarsson, Lavinia Cicortas

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CiteExportLink to record
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  • apa
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  • de-DE
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  • nn-NO
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