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The extracellular matrix-degrading protein ADAMTS5 is expressed in the nuclei of urothelial cells in healthy rats
Örebro University, School of Medical Sciences.
Department of Surgery, Institute of Clinical Sciences, Sahlgrenska Academy, University of Gothenburg, Gothenburg, Sweden.
Department of Urology, Institute of Clinical Sciences, Sahlgrenska Academy. University of Gothenburg, Gothenburg, Sweden.
Örebro University, School of Science and Technology.ORCID iD: 0000-0001-9713-2365
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2018 (English)In: Scandinavian journal of urology, ISSN 2168-1805, E-ISSN 2168-1813, Vol. 52, no 2, p. 139-142Article in journal (Refereed) Published
Abstract [en]

OBJECTIVE: The aim of this study was to investigate whether protein expression of the extracellular matrix-degrading protease ADAMTS5 can be demonstrated in the urinary bladder of healthy rats, and, if so, to determine the localization of this enzyme.

MATERIALS AND METHODS: The experiments were conducted with eight inbred male Sprague-Dawley rats. Immunohistochemistry was used to investigate the expression of ADAMTS5 in the urinary bladder. Negative controls were established by either excluding the primary antibody or applying the antibody after it had been preabsorbed with its immunogenic peptide. Confocal microscopy was used to visualize the distribution of ADAMTS5 in the urinary bladder tissue.

RESULTS: Immunoreactivity for ADAMTS5 was demonstrated in the urothelium and in the detrusor. This expression was localized not only in the cytoplasm, but also in the nuclei. Confocal microscopy corroborated these findings.

CONCLUSION: Expression of ADAMTS5 was demonstrated in the cytoplasm as well as in the nuclei of the urothelium and detrusor cells, suggesting that it may play a role at the transcriptional level.

Place, publisher, year, edition, pages
Informa Healthcare, 2018. Vol. 52, no 2, p. 139-142
Keywords [en]
ADAMTS5, aggrecanase, confocal laser scanning microscopy, immunohistochemistry, nuclear, rat urothelium
National Category
Urology and Nephrology
Identifiers
URN: urn:nbn:se:oru:diva-64508DOI: 10.1080/21681805.2017.1422015PubMedID: 29334289Scopus ID: 2-s2.0-85041138418OAI: oai:DiVA.org:oru-64508DiVA, id: diva2:1177378
Available from: 2018-01-25 Created: 2018-01-25 Last updated: 2018-08-28Bibliographically approved

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Delbro, DickScherbak, Nikolai

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