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Gas Chromatography-Mass Spectrometry Method Development of Fatty Acid Analysis for Forensic Analysis of Fire Debris
Örebro University, School of Science and Technology.
2017 (English)Independent thesis Basic level (degree of Bachelor), 10 credits / 15 HE creditsStudent thesis
Abstract [en]

Linseed oil possesses the capacity to self-ignite and thereby there is a forensic interest in analysing the compounds in the oil in order to potentially find signs of what has caused the fire. Out of forensic perspective a method validation and development for such an analysis is needed. Oils consists of fatty acids the majority of which are in the form of triglycerides. However, some free fatty acids exists in small amounts. When analyzing fatty acids with Gas chromatography (GC) it is crucial to transform the fatty acids into volatile compounds. This is usually done by converting them into their corresponding methyl esters. The method validation for two different methylation techniques, one acid catalysed and the other base catalysed, as well as one silylation technique implied that the base catalysed method was distinctively more efficient on the evaluated matrix of heated linseed oil extract in regards of peak intensity as well as number of detectable peaks. Silylation affirmed that the prevalence of free fatty acids in linseed oil are low and that these are successfully methylated, hence discarding the problematics surrounding the free fatty acids in the oil. Other limiting parameters were also studied. Overall the base catalysed method produced detectable fatty acid methyl esters (FAME:s) from the linseed oil extract, with conventional retention times and retention order harmonizing with mass spectral data from similar studies.

Place, publisher, year, edition, pages
2017. , p. 30
Keywords [en]
Method validation, FAME, silylation, fire debris, linseed oil.
National Category
Chemical Sciences
Identifiers
URN: urn:nbn:se:oru:diva-64644OAI: oai:DiVA.org:oru-64644DiVA, id: diva2:1178588
Subject / course
Chemistry
Supervisors
Examiners
Available from: 2018-01-30 Created: 2018-01-30 Last updated: 2019-03-04Bibliographically approved

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CiteExportLink to record
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Citation style
  • apa
  • harvard1
  • ieee
  • modern-language-association-8th-edition
  • vancouver
  • Other style
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Language
  • de-DE
  • en-GB
  • en-US
  • fi-FI
  • nn-NO
  • nn-NB
  • sv-SE
  • Other locale
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Output format
  • html
  • text
  • asciidoc
  • rtf