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RBBP6 increases radioresistance and serves as a therapeutic target for preoperative radiotherapy in colorectal cancer
Department of General Surgery, Shanghai General Hospital School of Medicine, Shanghai Jiao Tong University, Shanghai, People's Republic of China.
Department of General Surgery, Shanghai General Hospital School of Medicine, Shanghai Jiao Tong University, Shanghai, People's Republic of China.
Department of General Surgery, Shanghai General Hospital School of Medicine, Shanghai Jiao Tong University, Shanghai, People's Republic of China.
Department of General Surgery, Shanghai General Hospital School of Medicine, Shanghai Jiao Tong University, Shanghai, People's Republic of China.
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2018 (English)In: Cancer Science, ISSN 1347-9032, E-ISSN 1349-7006, Vol. 109, no 4, p. 1075-1087Article in journal (Refereed) Published
Abstract [en]

Radiotherapy (RT) can be used as preoperative treatment to downstage initially unresectable locally rectal carcinoma, but the radioresistance and recurrence remain significant problems. Retinoblastoma binding protein 6 (RBBP6) has been implicated in the regulation of cell cycle, apoptosis and chemoresistance both in vitro and in vivo. This study investigated whether the inhibition of RBBP6 expression would improve radiosensitivity in human colorectal cancer cells. After SW620 and HT29 cells were exposed to radiation, the levels of RBBP6 mRNA and protein increased over time in both two cells. Moreover, a significant reduction in clonogenic survival and a decrease in cell viability in parallel with an obvious increase in cell apoptosis were demonstrated in irradiated RBBP6-knockdown cells. Besides, transfection with RBBP6 shRNA improved levels of G2-M phase arrest which blocked the cells in a more radiosensitive period of the cell cycle. These observations indicated that cell cycle and apoptosis mechanisms may be connected with tumor cell survival following radiotherapy. In vivo, tumor growth rate of nude mice in RBBP6-knockdown group was significantly slower than that in other groups. These results indicated that RBBP6 overexpression could resist colorectal cancer cells against radiation by regulating cell cycle and apoptosis pathways, and inhibition of RBBP6 could enhance radiosensitivity of human colorectal cancer.

Place, publisher, year, edition, pages
Blackwell Publishing, 2018. Vol. 109, no 4, p. 1075-1087
Keywords [en]
Apoptosis, Cell cycle, Preoperative radiotherapy, RBBP6, Rectal cancer
National Category
Cancer and Oncology
Identifiers
URN: urn:nbn:se:oru:diva-64711DOI: 10.1111/cas.13516ISI: 000429411800020PubMedID: 29369481Scopus ID: 2-s2.0-85043476491OAI: oai:DiVA.org:oru-64711DiVA, id: diva2:1179961
Available from: 2018-02-02 Created: 2018-02-02 Last updated: 2018-09-20Bibliographically approved

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