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Novel, dynamic on-line analytical separation system for dissolution of drugs from poly(lactic acid) nanoparticles
Laboratory of Analytical Chemistry, Department of Chemistry, University of Helsinki, Helsinki, Finland.
Division of Pharmaceutical Technology, Faculty of Pharmacy, University of Helsinki, Helsinki, Finland.
Division of Pharmaceutical Technology, Faculty of Pharmacy, University of Helsinki, Helsinki, Finland.
Division of Pharmaceutical Technology, Faculty of Pharmacy, University of Helsinki, Helsinki, Finland.
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2010 (English)In: Journal of Pharmaceutical and Biomedical Analysis, ISSN 0731-7085, E-ISSN 1873-264X, Vol. 51, no 1, p. 125-130Article in journal (Refereed) Published
Abstract [en]

A novel method for investigating drug release in a dynamic manner from nanoparticles including, but not limited to, biodegradable poly(lactic acid) (PLA) is reported. The PLA nanoparticles were prepared by the nanoprecipitation method. Two poorly soluble drugs, beclomethasone dipropionate (BDP) and indomethacin, were encapsulated into PLA nanoparticles, and their dissolution from the nanoparticles were followed in a dynamic way. The on-line method comprised a short column (vessel) packed with the PLA nanoparticles, on-line connected to an analytical liquid chromatographic column via a multiport switching valve equipped with two loops. The system allowed monitoring of the drug release profiles in real time, and the conditions for the drug release could be precisely controlled and easily changed. The effects of solvent composition and temperature on the rate of dissolution of the drugs from the PLA nanoparticles were investigated. The system proved to be linear for the drugs tested over the concentration range 10-3000 ng (n = 6, R(2) = 0.999 and 0.997 for indomethacin and beclomethasone, respectively) and repeatable (RSD of peak areas <0.5%). The recoveries of the dissolution study were quantitative (120 and 103% for indomethacin and beclomethasone, respectively).

Place, publisher, year, edition, pages
Elsevier, 2010. Vol. 51, no 1, p. 125-130
Keywords [en]
Poly(lactic acid); Beclomethasone dipropionate; Indomethacin; Liquid chromatography; Dissolution; Drug release
National Category
Analytical Chemistry
Identifiers
URN: urn:nbn:se:oru:diva-65700DOI: 10.1016/j.jpba.2009.08.021ISI: 000270748100019PubMedID: 19744811Scopus ID: 2-s2.0-70349319347OAI: oai:DiVA.org:oru-65700DiVA, id: diva2:1189750
Note

Funding Agency:

Academy of Finland  SA114292  110429

Available from: 2018-03-12 Created: 2018-03-12 Last updated: 2019-03-04Bibliographically approved

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Hyötyläinen, Tuulia

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