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Spatial distribution of glycerophospholipids in the ocular lens
Laboratory of Molecular Structure Characterization, Institute of Microbiology, Academy of Sciences of the Czech Republic, Prague, Czech Republic; Division of Pharmaceutical Chemistry, Faculty of Pharmacy, University of Helsinki, Helsinki, Finland.
Laboratory of Molecular Structure Characterization, Institute of Microbiology, Academy of Sciences of the Czech Republic, Prague, Czech Republic; Department of Analytical Chemistry, Faculty of Science, Palacký University, Olomouc, Czech Republic.
VTT Technical Research Centre of Finland, Espoo, Finland.
Laboratory of Molecular Structure Characterization, Institute of Microbiology, Academy of Sciences of the Czech Republic, Prague, Czech Republic.
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2011 (English)In: PLoS ONE, ISSN 1932-6203, E-ISSN 1932-6203, Vol. 6, no 4, article id e19441Article in journal (Refereed) Published
Abstract [en]

Knowledge of the spatial distribution of lipids in the intraocular lens is important for understanding the physiology and biochemistry of this unique tissue and for gaining a better insight into the mechanisms underlying diseases of the lens. Following our previous study showing the spatial distribution of sphingolipids in the porcine lens, the current study used ultra performance liquid chromatography quadrupole time-of-flight mass spectrometry (UPLC-QTOFMS) to provide the whole lipidome of porcine lens and these studies were supplemented by matrix-assisted laser desorption ionization mass spectrometry imaging (MALDI MSI) of the lens using ultra-high resolution Fourier transform ion cyclotron resonance mass spectrometry (FTICR MS) to determine the spatial distribution of glycerophospholipids. Altogether 172 lipid species were identified with high confidence and their concentration was determined. Sphingomyelins, phosphatidylcholines, and phosphatidylethanolamines were the most abundant lipid classes. We then determined the spatial and concentration-dependent distributions of 20 phosphatidylcholines, 6 phosphatidylethanolamines, and 4 phosphatidic acids. Based on the planar molecular images of the lipids, we report the organization of fiber cell membranes within the ocular lens and suggest roles for these lipids in normal and diseased lenses.

Place, publisher, year, edition, pages
Public Library of Science , 2011. Vol. 6, no 4, article id e19441
National Category
Analytical Chemistry
Identifiers
URN: urn:nbn:se:oru:diva-65696DOI: 10.1371/journal.pone.0019441ISI: 000290024700172PubMedID: 21559377Scopus ID: 2-s2.0-79955762220OAI: oai:DiVA.org:oru-65696DiVA, id: diva2:1189761
Note

Funding Agencies:

Sigrid Juselius Foundation  

Finnish Eye Foundation  

Academy of Finland  122354 

University of Helsinki  2105060 

Ministry of Education, Youth, and Sports of the Czech Republic  LC07017 

Institutional Research Concept of the Institute of Microbiology  

Academy of Sciences of the Czech Republic  AV0Z50200510 

EC  

Czech Science Foundation  P206/10/P018 

Available from: 2018-03-12 Created: 2018-03-12 Last updated: 2018-03-13Bibliographically approved

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Hyötyläinen, Tuulia

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