Separation of nucleobases, nucleosides, and nucleotides using two zwitterionic silica-based monolithic capillary columns coupled with tandem mass spectrometryShow others and affiliations
2014 (English)In: Journal of Chromatography A, ISSN 0021-9673, E-ISSN 1873-3778, Vol. 1373, p. 90-96Article in journal (Refereed) Published
Abstract [en]
The capability of employing synthesized zwitterionic silica-based monolithic capillary columns (140 mm x 0.1 mm) for separation of highly polar and hydrophilic nucleobases, nucleosides, and nucleotides in hydrophilic interaction chromatography is reported. The suitability of the columns for on-line conjunction with electrospray tandem mass spectrometry was explored. Our results show that the grafted layer of zwitterionic monomer ([2-(methacryloyloxy)ethyl]-dimethyl-(3-sulfopropyl)-ammonium hydroxide or 2-methaciyloyloxyethyl phosphorylcholine) on the silica monolithic surface significantly improved the separation selectivity and reproducibility, as compared to the bare silica monolith. The stepwise elution from 90% to 70% of acetonitrile enabled separation of a complex sample mixture containing 21 compounds with a total analysis time less than 40 min.
Place, publisher, year, edition, pages
Elsevier, 2014. Vol. 1373, p. 90-96
Keywords [en]
HILIC separation; ESI-MS; Monolithic silica column; Nucleobase; Nucleoside; Nucleotide
National Category
Analytical Chemistry Biochemistry Molecular Biology
Identifiers
URN: urn:nbn:se:oru:diva-65683DOI: 10.1016/j.chroma.2014.11.015ISI: 000347265800011PubMedID: 25465366Scopus ID: 2-s2.0-84919387315OAI: oai:DiVA.org:oru-65683DiVA, id: diva2:1189778
Note
Funding Agencies:
Ministry of the Interior of the Czech Republic VG20112015021
Czech Science Foundation P106/12/0522
Academy of Sciences of the Czech Republic RVO: 68081715
Magnus Ehrnrooth Foundation
Academy of Finland 266342 257316
2018-03-122018-03-122025-02-20Bibliographically approved