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Separation of nucleobases, nucleosides, and nucleotides using two zwitterionic silica-based monolithic capillary columns coupled with tandem mass spectrometry
Institute of Analytical Chemistry of the ASCR, Brno, Czech Republic.
Faculty of Pharmacy, University of Helsinki, Finland.
Institute of Analytical Chemistry of the ASCR, Brno, Czech Republic.
Steno Diabetes Center, Gentofte, Denmark.
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2014 (English)In: Journal of Chromatography A, ISSN 0021-9673, E-ISSN 1873-3778, Vol. 1373, p. 90-96Article in journal (Refereed) Published
Abstract [en]

The capability of employing synthesized zwitterionic silica-based monolithic capillary columns (140 mm x 0.1 mm) for separation of highly polar and hydrophilic nucleobases, nucleosides, and nucleotides in hydrophilic interaction chromatography is reported. The suitability of the columns for on-line conjunction with electrospray tandem mass spectrometry was explored. Our results show that the grafted layer of zwitterionic monomer ([2-(methacryloyloxy)ethyl]-dimethyl-(3-sulfopropyl)-ammonium hydroxide or 2-methaciyloyloxyethyl phosphorylcholine) on the silica monolithic surface significantly improved the separation selectivity and reproducibility, as compared to the bare silica monolith. The stepwise elution from 90% to 70% of acetonitrile enabled separation of a complex sample mixture containing 21 compounds with a total analysis time less than 40 min.

Place, publisher, year, edition, pages
Elsevier, 2014. Vol. 1373, p. 90-96
Keywords [en]
HILIC separation; ESI-MS; Monolithic silica column; Nucleobase; Nucleoside; Nucleotide
National Category
Analytical Chemistry Biochemistry and Molecular Biology
Identifiers
URN: urn:nbn:se:oru:diva-65683DOI: 10.1016/j.chroma.2014.11.015ISI: 000347265800011PubMedID: 25465366Scopus ID: 2-s2.0-84919387315OAI: oai:DiVA.org:oru-65683DiVA, id: diva2:1189778
Note

Funding Agencies:

Ministry of the Interior of the Czech Republic  VG20112015021 

Czech Science Foundation  P106/12/0522 

Academy of Sciences of the Czech Republic  RVO: 68081715 

Magnus Ehrnrooth Foundation  

Academy of Finland  266342  257316 

Available from: 2018-03-12 Created: 2018-03-12 Last updated: 2018-03-14Bibliographically approved

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Hyötyläinen, Tuulia

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