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The lantibiotic gallidermin acts bactericidal against Staphylococcus epidermidis and Staphylococcus aureus and antagonizes the bacteria-induced proinflammatory responses in dermal fibroblasts
Örebro University, School of Medical Sciences.
Department of Oral Biology, Institute of Odontology, Malmö University, Malmö, Sweden; PEAS Research Institute, Linköping, Sweden.
Örebro University, School of Medical Sciences.
Örebro University, School of Medical Sciences.
2018 (English)In: MicrobiologyOpen, ISSN 2045-8827, E-ISSN 2045-8827Article in journal (Refereed) Epub ahead of print
Abstract [en]

Antimicrobial resistance needs to be tackled from new angles, and antimicrobial peptides could be future candidates for combating bacterial infections. This study aims to investigate in vitro the bactericidal effects of the lantibiotic gallidermin on Staphylococcus epidermidis and Staphylococcus aureus, possible cytotoxic effects and its impact on host-microbe interactions. Minimal inhibitory concentration (MIC) and minimal bactericidal concentration (MBC) of gallidermin were determined, and cytotoxicity and proinflammatory effects of gallidermin on fibroblasts, red blood cells (RBCs) and in whole blood were investigated. Both MIC and MBC for all four tested strains of S. epidermidis was 6.25 μg/ml. Both MIC and MBC for methicillin-sensitive S. aureus was 12.5 μg/ml and for methicillin-resistant S. aureus (MRSA) 1.56 μg/ml. Gallidermin displayed no cytotoxic effects on fibroblasts, only a high dose of gallidermin induced low levels of CXCL8 and interleukin-6. Gallidermin hemolyzed less than 1% of human RBCs, and did not induce reactive oxygen species production or cell aggregation in whole blood. In cell culture, gallidermin inhibited the cytotoxic effects of the bacteria and totally suppressed the bacteria-induced release of CXCL8 and interleukin-6 from fibroblasts. We demonstrate that gallidermin, expressing low cell cytotoxicity, is a promising candidate for treating bacterial infections caused by S. epidermidis and S. aureus, especially MRSA.

Place, publisher, year, edition, pages
John Wiley & Sons, 2018.
Keywords [en]
Streptococcus, antimicrobial resistance, bacteriocin, cytokines, fibroblasts, gallidermin
National Category
Immunology
Identifiers
URN: urn:nbn:se:oru:diva-65822DOI: 10.1002/mbo3.606PubMedID: 29536668OAI: oai:DiVA.org:oru-65822DiVA, id: diva2:1190676
Available from: 2018-03-15 Created: 2018-03-15 Last updated: 2018-09-05Bibliographically approved

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Bengtsson, TorbjörnKhalaf, HazemPalm, Eleonor

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