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Divergent metabolic outcomes arising from targeted manipulation of the gut microbiota in diet-induced obesity
Alimentary Pharmabiotic Centre, University College Cork, Cork, Ireland; Alimentary Health Ltd., Cork, Ireland.
Alimentary Pharmabiotic Centre, University College Cork, Cork, Ireland; Teagasc Food Research Centre, Fermoy, Ireland.
Alimentary Pharmabiotic Centre, University College Cork, Cork, Ireland.
Teagasc Food Research Centre, Fermoy, Ireland.
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2013 (English)In: Gut, ISSN 0017-5749, E-ISSN 1468-3288, Vol. 62, no 2, p. 220-226Article in journal (Refereed) Published
Abstract [en]

OBJECTIVE: The gut microbiota is an environmental regulator of fat storage and adiposity. Whether the microbiota represents a realistic therapeutic target for improving metabolic health is unclear. This study explored two antimicrobial strategies for their impact on metabolic abnormalities in murine diet-induced obesity: oral vancomycin and a bacteriocin-producing probiotic (Lactobacillus salivarius UCC118 Bac(+)).

DESIGN: Male (7-week-old) C57BL/J6 mice (9-10/group) were fed a low-fat (lean) or a high-fat diet for 20 weeks with/without vancomycin by gavage at 2 mg/day, or with L. salivarius UCC118Bac(+) or the bacteriocin-negative derivative L. salivarius UCC118Bac(-) (each at a dose of 1×10(9) cfu/day by gavage). Compositional analysis of the microbiota was by 16S rDNA amplicon pyrosequencing.

RESULTS: Analysis of the gut microbiota showed that vancomycin treatment led to significant reductions in the proportions of Firmicutes and Bacteroidetes and a dramatic increase in Proteobacteria, with no change in Actinobacteria. Vancomycin-treated high-fat-fed mice gained less weight over the intervention period despite similar caloric intake, and had lower fasting blood glucose, plasma TNFα and triglyceride levels compared with diet-induced obese controls. The bacteriocin-producing probiotic had no significant impact on the proportions of Firmicutes but resulted in a relative increase in Bacteroidetes and Proteobacteria and a decrease in Actinobacteria compared with the non-bacteriocin-producing control. No improvement in metabolic profiles was observed in probiotic-fed diet-induced obese mice.

CONCLUSION: Both vancomycin and the bacteriocin-producing probiotic altered the gut microbiota in diet-induced obese mice, but in distinct ways. Only vancomycin treatment resulted in an improvement in the metabolic abnormalities associated with obesity thereby establishing that while the gut microbiota is a realistic therapeutic target, the specificity of the antimicrobial agent employed is critical.

Place, publisher, year, edition, pages
BMJ Publishing Group Ltd, 2013. Vol. 62, no 2, p. 220-226
National Category
Medical and Health Sciences Gastroenterology and Hepatology
Identifiers
URN: urn:nbn:se:oru:diva-65932DOI: 10.1136/gutjnl-2011-300705ISI: 000313264400007PubMedID: 22345653Scopus ID: 2-s2.0-84872112919OAI: oai:DiVA.org:oru-65932DiVA, id: diva2:1192019
Note

Erratum in Gut. 2013 Feb;62(2):226.

Available from: 2018-03-21 Created: 2018-03-21 Last updated: 2019-04-24Bibliographically approved

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Marques, Tatiana M.

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