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Streptozotocin-induced type-1-diabetes disease onset in Sprague-Dawley rats is associated with an altered intestinal microbiota composition and decreased diversity
Alimentary Pharmabiotic Centre, Biosciences Institute, University College Cork, Co. Cork, Ireland; Teagasc Food Research Centre, Food Biosciences Department, Moorepark, Fermoy, Co. Cork, Ireland; Department of Microbiology, University College Cork, Co. Cork, Ireland.
Alimentary Pharmabiotic Centre, Biosciences Institute, University College Cork, Co. Cork, Ireland; Teagasc Food Research Centre, Food Biosciences Department, Moorepark, Fermoy, Co. Cork, Ireland; Department of Microbiology, University College Cork, Co. Cork, Ireland.
Alimentary Pharmabiotic Centre, Biosciences Institute, University College Cork, Co. Cork, Ireland; Teagasc Food Research Centre, Food Biosciences Department, Moorepark, Fermoy, Co. Cork, Ireland.
Alimentary Pharmabiotic Centre, Biosciences Institute, University College Cork, Co. Cork, Ireland.
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2015 (English)In: Microbiology, ISSN 1350-0872, E-ISSN 1465-2080, Vol. 161, no Pt 1, p. 182-93Article in journal (Refereed) Published
Abstract [en]

There is a growing appreciation that microbiota composition can significantly affect host health and play a role in disease onset and progression. This study assessed the impact of streptozotocin (STZ)-induced type-1-diabetes (T1D) on intestinal microbiota composition and diversity in Sprague-Dawley rats, compared with healthy controls over time. T1D was induced by injection of a single dose (60 mg STZ kg(-1)) of STZ, administered via the intraperitoneal cavity. Total DNA was isolated from faecal pellets at weeks 0 (pre-STZ injection), 1, 2 and 4 and from caecal content at week 5 from both healthy and T1D groups. High-throughput 16S rRNA sequencing was employed to investigate intestinal microbiota composition. The data revealed that although intestinal microbiota composition between the groups was similar at week 0, a dramatic impact of T1D development on the microbiota was apparent post-STZ injection and for up to 5 weeks. Most notably, T1D onset was associated with a shift in the Bacteroidetes : Firmicutes ratio (P<0.05), while at the genus level, increased proportions of lactic acid producing bacteria such as Lactobacillus and Bifidobacterium were associated with the later stages of T1D progression (P<0.05). Coincidently, T1D increased caecal lactate levels (P<0.05). Microbial diversity was also reduced following T1D (P<0.05). Principle co-ordinate analyses demonstrated temporal clustering in T1D and control groups with distinct separation between groups. The results provide a comprehensive account of how T1D is associated with an altered intestinal microbiota composition and reduced microbial diversity over time.

Place, publisher, year, edition, pages
London, UK: The Microbiology Society , 2015. Vol. 161, no Pt 1, p. 182-93
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Medical and Health Sciences Microbiology
Identifiers
URN: urn:nbn:se:oru:diva-65930DOI: 10.1099/mic.0.082610-0ISI: 000356647700017PubMedID: 25370749Scopus ID: 2-s2.0-84941736218OAI: oai:DiVA.org:oru-65930DiVA, id: diva2:1192021
Note

Funding Agencies:

Teagasc Walsh Fellowship Scheme 

Science Foundation of Ireland, 07/CE/B1368 

Available from: 2018-03-21 Created: 2018-03-21 Last updated: 2018-03-22Bibliographically approved

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Marques, Tatiana M.

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