oru.sePublications
Change search
CiteExportLink to record
Permanent link

Direct link
Cite
Citation style
  • apa
  • ieee
  • modern-language-association-8th-edition
  • vancouver
  • Other style
More styles
Language
  • de-DE
  • en-GB
  • en-US
  • fi-FI
  • nn-NO
  • nn-NB
  • sv-SE
  • Other locale
More languages
Output format
  • html
  • text
  • asciidoc
  • rtf
Ambient hypoxia enhances the loss of muscle mass after extensive injury
Département Environnements Opérationnels, Institut de Recherche Biomédicale des Armées, Antenne de la Tronche, La Tronche, France; Center for Muscle Biology, Department of Physiology, University of Kentucky, Lexington, KY, United States.ORCID iD: 0000-0002-5322-4150
Département Environnements Opérationnels, Institut de Recherche Biomédicale des Armées, Antenne de la Tronche, La Tronche, France; Ecole du Val-de-Grâce, Paris, France.
Département Environnements Opérationnels, Institut de Recherche Biomédicale des Armées, Antenne de la Tronche, La Tronche, France.
Pôle Génomique, Institut de Recherche Biomédicale des Armées, La Tronche, France.
Show others and affiliations
2014 (English)In: Pflügers Archiv: European Journal of Physiology, ISSN 0031-6768, E-ISSN 1432-2013, Vol. 466, no 3, p. 587-598Article in journal (Refereed) Published
Abstract [en]

Hypoxia induces a loss of skeletal muscle mass and alters myogenesis in vitro, but whether it affects muscle regeneration in vivo following injury remains to be elucidated. We hypothesized that hypoxia would impair the recovery of muscle mass during regeneration. To test this hypothesis, the soleus muscle of female rats was injured by notexin and allowed to recover for 3, 7, 14, and 28 days under normoxia or hypobaric hypoxia (5,500 m) conditions. Hypoxia impaired the formation and growth of new myofibers and enhanced the loss of muscle mass during the first 7 days of regeneration, but did not affect the final recovery of muscle mass at 28 days. The impaired regeneration under hypoxic conditions was associated with a blunted activation of mechanical target of rapamycin (mTOR) signaling as assessed by p70(S6K) and 4E-BP1 phosphorylation that was independent of Akt activation. The decrease in mTOR activity with hypoxia was consistent with the increase in AMP-activated protein kinase activity, but not related to the change in regulated in development and DNA response 1 protein content. Hypoxia increased the mRNA levels of the atrogene muscle ring finger-1 after 7 days of regeneration, though muscle atrophy F box transcript levels remained unchanged. The increase in MyoD and myogenin mRNA expression with regeneration was attenuated at 7 days with hypoxia. In conclusion, our results support the notion that the enhanced loss of muscle mass observed after 1 week of regeneration under hypoxic conditions could mainly result from the impaired formation and growth of new fibers resulting from a reduction in protein synthesis and satellite cell activity.

Place, publisher, year, edition, pages
Springer, 2014. Vol. 466, no 3, p. 587-598
Keyword [en]
Muscle regeneration, Akt/mTOR pathway, AMPK, Satellite cells, MRF
National Category
Medical and Health Sciences Physiology
Identifiers
URN: urn:nbn:se:oru:diva-66014DOI: 10.1007/s00424-013-1336-7ISI: 000331719400021PubMedID: 23974966Scopus ID: 2-s2.0-84896721442OAI: oai:DiVA.org:oru-66014DiVA, id: diva2:1192499
Note

Funding Agencies:

Ministere de I'Enseignement Superieur et de la Recherche 

Association Francaise contre les Myopathies, 13955

Available from: 2018-03-22 Created: 2018-03-22 Last updated: 2018-03-26Bibliographically approved

Open Access in DiVA

No full text in DiVA

Other links

Publisher's full textPubMedScopus

Authority records BETA

Chaillou, Thomas

Search in DiVA

By author/editor
Chaillou, Thomas
In the same journal
Pflügers Archiv: European Journal of Physiology
Medical and Health SciencesPhysiology

Search outside of DiVA

GoogleGoogle Scholar

doi
pubmed
urn-nbn

Altmetric score

doi
pubmed
urn-nbn
Total: 24 hits
CiteExportLink to record
Permanent link

Direct link
Cite
Citation style
  • apa
  • ieee
  • modern-language-association-8th-edition
  • vancouver
  • Other style
More styles
Language
  • de-DE
  • en-GB
  • en-US
  • fi-FI
  • nn-NO
  • nn-NB
  • sv-SE
  • Other locale
More languages
Output format
  • html
  • text
  • asciidoc
  • rtf