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Platelet adhesion changes during storage studied with a novel method using flow cytometry and protein-coated beads
Department of Clinical and Experimental Medicine, Division of Transfusion Medicine, Faculty of Health Sciences, Linköping University, Linköping, Sweden; Department of Clinical Immunology and Transfusion Medicine, County Council of Östergötland, Linköping, Sweden; Department of Clinical Chemistry, County Council of Östergötland, Linköping, Sweden.
Department of Clinical and Experimental Medicine, Division of Clinical Chemistry, Faculty of Health Sciences, Linköping University, Linköping, Sweden.
Department of Clinical and Experimental Medicine, Division of Clinical Chemistry, Faculty of Health Sciences, Linköping University, Linköping, Sweden.ORCID iD: 0000-0003-0460-4744
Linkoping Univ, Fac Hlth Sci, Dept Clin & Expt Med, Div Clin Chem, SE-58185 Linkoping, Sweden..
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2015 (English)In: Platelets, ISSN 0953-7104, E-ISSN 1369-1635, Vol. 26, no 2, p. 177-185Article in journal (Refereed) Published
Abstract [en]

The aim of the present study was to set up and evaluate a novel method for studies of platelet adhesion and activation in blood and platelet suspensions such as platelet concentrate (PC) samples using protein-coated polystyrene beads and flow cytometry. To demonstrate its usefulness, we studied PCs during storage. PCs were prepared by aphaeresis technique (n = 7). Metabolic variables and platelet function was measured on day 1, 5, 7 and 12 of storage. Spontaneous and TRAP-6-induced adhesion to fibrinogen-and collagen-coated beads was analyzed by flow cytometry. P-selectin and phosphatidyl serine (PS) expression was assessed on platelets bound to beads as well as on non-adherent platelets. Platelet adhesion to fibrinogen beads had increased by day 12 and adhesion to collagen beads at day 7 of storage (p<0.05). TRAP-6 stimulation significantly increased the platelet adhesion to fibrinogen beads (p<0.05) as well as the P-selectin and PS exposure on platelets bound to beads (p<0.01) during the first 7 days of storage, but by day 12, significant changes were no longer induced by TRAP-6 stimulation. We demonstrate that our adhesion assay using protein-coated polystyrene beads can be used to assess the adhesion properties of platelets during storage without the addition of red blood cells. Therefore it may offer a useful tool for future studies of platelet adhesive capacity in transfusion medicine and other settings.

Place, publisher, year, edition, pages
Taylor & Francis, 2015. Vol. 26, no 2, p. 177-185
Keywords [en]
Adhesion molecules, blood products, platelet glycoproteins, transfusion medicine
National Category
Cell Biology
Identifiers
URN: urn:nbn:se:oru:diva-66031DOI: 10.3109/09537104.2014.891728ISI: 000351740700012PubMedID: 24679340Scopus ID: 2-s2.0-84922801860OAI: oai:DiVA.org:oru-66031DiVA, id: diva2:1192613
Available from: 2018-03-22 Created: 2018-03-22 Last updated: 2018-03-27Bibliographically approved

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