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Whole-Genome Sequencing of Emerging Invasive Neisseria meningitidis Serogroup W in Sweden
Örebro University, School of Medical Sciences. Department of Laboratory Medicine, Faculty of Health and Medical Sciences, Örebro University, Örebro, Sweden.
Department of Laboratory Medicine, Örebro University Hospital, Örebro, Sweden.
Örebro University, School of Medical Sciences. Department of Laboratory Medicine, Örebro University Hospital, Örebro, Sweden.
Department of Laboratory Medicine, Örebro University Hospital, Örebro, Sweden.
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2018 (English)In: Journal of Clinical Microbiology, ISSN 0095-1137, E-ISSN 1098-660X, Vol. 56, no 4, article id e01409-17Article in journal (Refereed) Published
Abstract [en]

Invasive disease caused by Neisseria meningitidis serogroup W (MenW) has historically had a low incidence in Sweden, with an average incidence of 0.03 case/100,000 population from 1995 to 2014. In recent years, a significant increase in the incidence of MenW has been noted in Sweden, to an average incidence of 0.15 case/100,000 population in 2015 to 2016. In 2017 (1 January to 30 June), 33% of invasive meningococcal disease cases (7/21 cases) were caused by MenW. In the present study, all invasive MenW isolates from Sweden collected in 1995 to June 2017 (n = 86) were subjected to whole-genome sequencing to determine the population structure and to compare isolates from Sweden with historical and international cases. The increase of MenW in Sweden was determined to be due to isolates belonging to the South American sublineage of MenW clonal complex 11, namely, the novel U.K. 2013 lineage. This lineage was introduced in Sweden in 2013 and has since been the dominant lineage of MenW.

Place, publisher, year, edition, pages
American Society for Microbiology , 2018. Vol. 56, no 4, article id e01409-17
Keywords [en]
CC11, Neisseria meningitidis, invasive meningococcal disease, serogroup W, whole-genome sequencing
National Category
Infectious Medicine
Identifiers
URN: urn:nbn:se:oru:diva-66477DOI: 10.1128/JCM.01409-17ISI: 000429718700010PubMedID: 29321195Scopus ID: 2-s2.0-85044717735OAI: oai:DiVA.org:oru-66477DiVA, id: diva2:1197606
Note

Funding Agencies:

Örebro County Council Research Committee

Nyckelfonden

Wellcome Trust

European Union

Available from: 2018-04-13 Created: 2018-04-13 Last updated: 2024-05-06Bibliographically approved
In thesis
1. Exploring genomic and phenotypic differences in Neisseria meningitidis: understanding carriage and invasive disease
Open this publication in new window or tab >>Exploring genomic and phenotypic differences in Neisseria meningitidis: understanding carriage and invasive disease
2024 (English)Doctoral thesis, comprehensive summary (Other academic)
Abstract [en]

Neisseria meningitidis can colonise the nasopharynx in humans and is also the cause of invasive meningococcal disease (IMD), which often presents as septicaemia and meningitis with high mortality rates. Invasive disease is often associated with specific capsular serogroups and clonal complexes (CC). In Sweden, serogroups Y and W have had a high incidence in recent years, but were previously considered rare causes of IMD, suggesting a change in the virulence potential of these serogroups. Currently, no specific genes exist that can reliably predict whether an N. meningitidis isolate will result in invasive disease or remain in the carriage state. Genetically similar isolates can be found during carriage and IMD, and it is more common for the carriage isolates to lack a capsule. The aim of this thesis was to investigate how genetic and phenotypic differences in N. meningitidis, can affect the virulence and the transition from a carriage state to invasive disease.

The results indicate that the increase of serogroup W in Sweden is due to a specific lineage of CC11. This CC is rarely found among carriers and is considered highly virulent. Infections in transgenic mice with serogroup W CC11 isolates showed a greater virulence compared to serogroup Y isolates from other CCs. Although both serogroups are common causes of IMD in Sweden, they differ in virulence in transgenic mice. A genome-wide association study comparing carriage and invasive isolates, revealed that there were genetic variants in genes associated with virulence between these isolates. Among these variants were pilE/pilS, which are involved in the type IV pili. Comparison of pilE gene expression between carriage and invasive isolates showed no significant difference between these isolates. However, a difference in the class of the PilE protein was found between invasive and carriage isolates. Further research is needed to understand the impact of these genetic variations on the transition from carriage to invasive disease, also considering how factors in the human host and the environment that may contribute to the development of invasive disease.

Place, publisher, year, edition, pages
Örebro: Örebro University, 2024. p. 81
Series
Örebro Studies in Medicine, ISSN 1652-4063 ; 292
Keywords
Neisseria meningitidis, invasive meningococcal disease, carriage, virulence, whole genome sequencing, genome wide association study
National Category
Other Basic Medicine
Identifiers
urn:nbn:se:oru:diva-112752 (URN)9789175295572 (ISBN)9789175295589 (ISBN)
Public defence
2024-05-31, Örebro universitet, Campus USÖ, hörsal X3, Södra Grev Rosengatan 32, Örebro, 09:00 (Swedish)
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Available from: 2024-04-02 Created: 2024-04-02 Last updated: 2024-05-27Bibliographically approved

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Eriksson, LorraineJacobsson, SusanneStenmark, Bianca

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