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Evaluation of Congo Red Staining in Degenerating Porcine Photoreceptors In Vitro: Protective Effects by Structural and Trophic Support
Department of Chemistry and Biomedicine, Linnaeus University, Kalmar, Sweden.
Örebro University, School of Medical Sciences.
Department of Clinical Neuroscience, Section for Ophthalmology and Vision, St. Erik Eye Hospital, Karolinska Institutet, Stockholm, Sweden.
Department of Science, Kristianstad University, Kristianstad, Sweden.
2018 (English)In: Journal of Histochemistry and Cytochemistry, ISSN 0022-1554, E-ISSN 1551-5044, Vol. 66, no 9, p. 631-641Article in journal (Refereed) Published
Abstract [en]

Congo red (CR) is a histological stain used for the detection of extracellular amyloids mediating various neurodegenerative diseases. Given that damaged photoreceptors appear to degenerate similarly to other nerve cells, CR staining was evaluated in experimentally injured porcine retina. CR staining appeared mostly as discrete cytosolic deposits with no obvious plaque formation during the investigated time period. Increases of CR labeling coincided temporally with the known accumulation of mislocalized opsins and increases of cell death. Coculture, either with human retinal pigment epithelium (ARPE) or human neural progenitor (ReN) cells, was accompanied by a significant reduction of CR labeling. Of particular interest was the reduction of CR labeling in cone photoreceptors, which are important for the perception of color and fine details and afflicted in age-related macular degeneration (AMD). Electron microscopy revealed inclusions in the inner segment, cell body, and occasionally synaptic terminals of photoreceptor cells in cultured specimens. Closer examinations indicated the presence of different types of inclusions resembling protein aggregates as well as inclusion bodies. The current results indicate that injury-related response resulted in accumulation of CR deposits in photoreceptor cells, and that trophic and/or structural support attenuated this response.

Place, publisher, year, edition, pages
Sage Publications, 2018. Vol. 66, no 9, p. 631-641
Keywords [en]
Congo red, electron microscopy, fluorescence microscopy, photoreceptor, retina
National Category
Neurosciences Cell and Molecular Biology
Identifiers
URN: urn:nbn:se:oru:diva-66436DOI: 10.1369/0022155418768222ISI: 000443345700002PubMedID: 29624116Scopus ID: 2-s2.0-85045027011OAI: oai:DiVA.org:oru-66436DiVA, id: diva2:1198232
Note

Funding Agencies:

Faculty of Medical Sciences at Örebro University  

Olle Engkvist Foundation  

Ögonfonden  

Crown Princess Margaretas Committee for the Blind  

Edwin Jordan Foundation  

Sven and Dagmar Salen Foundation 

Available from: 2018-04-17 Created: 2018-04-17 Last updated: 2018-09-12Bibliographically approved

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