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Testis transcriptome alterations in zebrafish (Danio rerio) with reduced fertility due to developmental exposure to 17 alpha-ethinyl estradiol
School of Natural Sciences, Technology and Environmental Studies, Södertörn University, Huddinge, Sweden.
School of Natural Sciences, Technology and Environmental Studies, Södertörn University, Huddinge, Sweden; Örebro Life Science Center, School of Science and Technology, Örebro University, Örebro, Sweden.
School of Natural Sciences, Technology and Environmental Studies, Södertörn University, Huddinge, Sweden; Örebro Life Science Center, School of Science and Technology, Örebro University, Örebro, Sweden.
School of Natural Sciences, Technology and Environmental Studies, Södertörn University, Huddinge, Sweden.
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2018 (English)In: General and Comparative Endocrinology, ISSN 0016-6480, E-ISSN 1095-6840, Vol. 262, p. 44-58Article in journal (Refereed) Published
Abstract [en]

17 alpha-Ethinylestradiol (EE2) is a ubiquitous aquatic contaminant shown to decrease fish fertility at low concentrations, especially in fish exposed during development. The mechanisms of the decreased fertility are not fully understood. In this study, we perform transcriptome analysis by RNA sequencing of testes from zebrafish with previously reported lowered fertility due to exposure to low concentrations of EE2 during development. Fish were exposed to 1.2 and 1.6 ng/L (measured concentration; nominal concentrations 3 and 10 ng/L) of EE2 from fertilization to 80 days of age, followed by 82 days of remediation in clean water. RNA sequencing analysis revealed 249 and 16 genes to be differentially expressed after exposure to 1.2 and 1.6 ng/L, respectively; a larger inter-sample variation was noted in the latter. Expression of 11 genes were altered by both exposures and in the same direction. The coding sequences most affected could be categorized to the putative functions cell signalling, proteolysis, protein metabolic transport and lipid metabolic process. Several homeobox transcription factors involved in development and differentiation showed increased expression in response to EE2 and differential expression of genes related to cell death, differentiation and proliferation was observed. In addition, several genes related to steroid synthesis, testis development and function were differentially expressed. A number of genes associated with spermatogenesis in zebrafish and/or mouse were also found to be differentially expressed. Further, differences in non-coding sequences were observed, among them several differentially expressed miRNA that might contribute to testis gene regulation at post-transcriptional level. This study has generated insights of changes in gene expression that accompany fertility alterations in zebrafish males that persist after developmental exposure to environmental relevant concentrations of EE2 that persist followed by clean water to adulthood. Hopefully, this will generate hypotheses to test in search for mechanistic explanations.

Place, publisher, year, edition, pages
Academic Press, 2018. Vol. 262, p. 44-58
National Category
Endocrinology and Diabetes
Identifiers
URN: urn:nbn:se:oru:diva-66918DOI: 10.1016/j.ygcen.2018.03.011ISI: 000430995100006PubMedID: 29526718Scopus ID: 2-s2.0-85044314644OAI: oai:DiVA.org:oru-66918DiVA, id: diva2:1205939
Note

Funding Agencies:

Swedish Baltic Sea Foundation  1556/42/2011 

Stockholm County Council  806/3.1.1/2014 

National Genomics Infrastructure (NGI)  

RFI/VR  

Science for Life Laboratory, Sweden  

Available from: 2018-05-15 Created: 2018-05-15 Last updated: 2018-05-15Bibliographically approved

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Olsson, Per-Erik

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