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Difference in the action spectra for UVR8 monomerisation and HY5 transcript accumulation in Arabidopsis
Institute of Molecular, Cell and Systems Biology, College of Medical, Veterinary and Life Sciences, Bower Building, University of Glasgow, Glasgow, UK.
Örebro University, School of Science and Technology. Institute of Molecular, Cell and Systems Biology, College of Medical, Veterinary and Life Sciences, Bower Building, University of Glasgow, Glasgow, UK. (Molekylär biokemi)ORCID iD: 0000-0002-8834-4064
Department of Plant Breeding, Swedish University of Agricultural Sciences, Alnarp, Sweden; School of Science & Technology, Örebro Life Science Center, Örebro University, Örebro, Sweden.
Department of Chemistry and Molecular Biology, University of Gothenburg, Gothenburg, Sweden.
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2018 (English)In: Photochemical and Photobiological Sciences, ISSN 1474-905X, E-ISSN 1474-9092, Vol. 17, no 8, p. 1108-1117Article in journal (Refereed) Published
Abstract [en]

The photoreceptor UV RESISTANCE LOCUS 8 (UVR8) activates photomorphogenic responses when plants are exposed to ultraviolet-B (UVB) light. However, whereas the absorption spectrum of UVR8 peaks at 280 nm, action spectra for several photomorphogenic UV-B responses show maximal photon effectiveness at 290-300 nm. To investigate this apparent discrepancy we measured the effectiveness of UV wavelengths in initiating two responses in Arabidopsis: photoconversion of homodimeric UVR8 into the monomeric form, which is active in signaling, and accumulation of transcripts of the ELONGATED HYPOCOTYL 5 (HY5) transcription factor, which has a key role in UVR8-mediated responses. When purified UVR8 or Arabidopsis leaf extracts were exposed to UV light monomerisation was maximal at approximately 280 nm, which correlates with the UVR8 absorption spectrum. When intact plants were exposed to UV, monomerisation was most strongly initiated at approximately 290 nm, and this shift in maximal effectiveness could be explained by strong absorption or reflectance at 280 nm by leaf tissue. Notably, the action spectrum for accumulation of HY5 transcripts in the same leaf tissue samples used to assay UVR8 dimer/monomer status peaked at approximately 300 nm. Possible reasons for the difference in maximal photon effectiveness of UVR8 monomerisation and HY5 transcript accumulation in leaf tissue are discussed.

Place, publisher, year, edition, pages
Royal Society of Chemistry, 2018. Vol. 17, no 8, p. 1108-1117
National Category
Plant Biotechnology Biochemistry and Molecular Biology
Research subject
Biochemistry
Identifiers
URN: urn:nbn:se:oru:diva-67649DOI: 10.1039/C8PP00138CISI: 000441153300013PubMedID: 29993086Scopus ID: 2-s2.0-85051272104OAI: oai:DiVA.org:oru-67649DiVA, id: diva2:1230133
Funder
Knowledge Foundation, 20130164Swedish Research Council FormasCarl Tryggers foundation
Note

Funding Agencies:

Consejo Nacional de Ciencia y Tecnologia (CONACYT)  

UK Biotechnology and Biological Sciences Research Council PhD studentship (University of Glasgow)  

Sven and Lily Lawski's Foundation for Scientific Research (University of Örebro) 

Faculty for Business, Science, and Technology at Örebro University

EU COST action 'UV4Growth'  FA0906 

University of Glasgow 

Available from: 2018-07-03 Created: 2018-07-03 Last updated: 2018-08-22Bibliographically approved

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O'Hara, AndrewStrid, Åke

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