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Microbial binding of per- and polyfluorinated alkyl substances (PFASs): - Analysis of PFASs in microbes with ultra-performance liquid chromatography – tandem mass spectrometry (UPLC-MS/MS)
Örebro University, School of Science and Technology.
2018 (English)Independent thesis Basic level (degree of Bachelor), 10 credits / 15 HE creditsStudent thesis
Abstract [en]

Per- and polyfluorinated alkyl substances (PFASs) belong to a large group of man-made chemicals that pollute the environment. Perfluorooctane sulfonic acid (PFOS) and perfluorooctanoic acid (PFOA) are the most commonly found PFASs. The pollution of PFASs can be caused among others by using of aqueous fire-fighting foams (AFFFs). PFASs are persistent compounds; that can travel long distances and bioaccumulate in biota. There are several exposure routes for PFASs, but the most common are via food and drinking water. A possible way for PFASs to enter the food chain is by adsorption to microbes. In this project, binding of PFASs to three gram-negative bacteria, Eschericha coli, Acidovorax delafieldii and Pseudomonas nitroreducens, was assessed. Microbes were exposed for fluorinated compounds in environmental water samples and a PFAS-11 solution with 11 PFAS substances prepared in the laboratory. The binding seems to be preferential to the most abundant compounds, PFOS, since the second most abundant compound in the samples was PFHxS with concentrations at one third of the PFOS concentration but nonetheless PFHxS was not detected in any of the samples.

The binding of mainly one PFAS was identified; PFOS was bound at highest concentrations in E. coli treated with both environmental water sample and a PFAS-11 solution. Low concentrations of FOSA and PFDoDS were identified in E. coli and PFNA in A. delafieldii. Only PFOS was detected in P. nitroreducens. The concentrations of other PFASs were below their respective method detection limits.

Place, publisher, year, edition, pages
2018. , p. 31
Keywords [en]
PFASs, microbes, microbial binding, PFOS, PFHxS, AFFFs.
National Category
Chemical Sciences
Identifiers
URN: urn:nbn:se:oru:diva-69080OAI: oai:DiVA.org:oru-69080DiVA, id: diva2:1251646
Subject / course
Chemistry
Supervisors
Examiners
Available from: 2018-09-27 Created: 2018-09-27 Last updated: 2018-09-27Bibliographically approved

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Microbial binding of per- and polyfluorinated alkyl substances (PFASs)(465 kB)81 downloads
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CiteExportLink to record
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Citation style
  • apa
  • ieee
  • modern-language-association-8th-edition
  • vancouver
  • Other style
More styles
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  • de-DE
  • en-GB
  • en-US
  • fi-FI
  • nn-NO
  • nn-NB
  • sv-SE
  • Other locale
More languages
Output format
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  • asciidoc
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