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Spatial mapping of affinity changes for the integrin LFA-1 during cell migration using clusters identified based on local density
Section of Immunology, Department of Experimental Medical Science, Lund University, Lund, Sweden.
Department of Biochemistry and Structural Biology, Lund University, Lund, Sweden.
Section of Immunology, Department of Experimental Medical Science, Lund University, Lund, Sweden.
Örebro University, School of Medical Sciences. Section of Immunology, Department of Experimental Medical Science, Lund University, Lund, Sweden.
2019 (English)In: Journal of Biophotonics, ISSN 1864-063X, E-ISSN 1864-0648, Vol. 12, no 3, article id e201800080Article in journal (Refereed) Published
Abstract [en]

Localization microscopy methods like Stochastic Optical Reconstruction Microscopy (STORM) are very well suited for exploring clustering of proteins, as the data inherently provide a list of molecular coordinates. Here we use state-of-art cluster analysis algorithms (DBSCAN) to explore the clustering behaviour of different affinity forms of the integrin LFA-1. It has been suggested that LFA-1 may form clusters, in order to increase the avidity to ICAM-1. However, this hypothesis still seems to be controversial. In this study, we found, variations in clustering behaviour among the different affinity forms of LFA-1 in migrating T-cells. We found that panLFA-1 is located in clusters throughout the polarized cell on ICAM-1, with an increased density of molecules and clusters in the mid area and rear of the cell, whereas the intermediate and high affinity form of LFA-1 showed an increased number in the mid area of a migrating cell and the high affinity form of LFA-1 in the front and rear. Together, these data suggest that, in addition to LFA-1 conformation, protein clustering might play a role in controlling cell-substrate adhesion on ICAM-1. By applying the cluster analysis algorithm DBSCAN to localization microscopy data, integrin clusters could be identified and different cluster parameters could be quantified.

Place, publisher, year, edition, pages
Wiley - VCH Verlag Gmbh , 2019. Vol. 12, no 3, article id e201800080
Keywords [en]
DBSCAN, LFA-1, Localization microscopy, STORM, T-lymphocyte, cluster analysis, integrins
National Category
Medical Biotechnology (with a focus on Cell Biology (including Stem Cell Biology), Molecular Biology, Microbiology, Biochemistry or Biopharmacy)
Identifiers
URN: urn:nbn:se:oru:diva-69117DOI: 10.1002/jbio.201800080ISI: 000462683300001PubMedID: 30267470Scopus ID: 2-s2.0-85056079021OAI: oai:DiVA.org:oru-69117DiVA, id: diva2:1252296
Funder
Swedish Research Council, K2010-80P-21592-01-4 K2010-80X-215917-01-4Available from: 2018-10-01 Created: 2018-10-01 Last updated: 2019-06-19Bibliographically approved

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