MS-based lipidomics of human blood plasma: a community-initiated position paper to develop accepted guidelinesDepartment of Biochemistry, YLL School of Medicine, National University of Singapore, Singapore.
Departments of Pharmacology and Chemistry and Biochemistry, School of Medicine, University of California at San Diego, La Jolla, CA, USA.
Lipidomics Consulting Ltd., Esbo, Finland.
Barshop Institute for Longevity and Aging Studies and Department of Medicine-Diabetes, University of Texas Health Science Center at San Antonio, San Antonio, TX, USA.
Laboratory for Metabolomics, RIKEN Center for Integrative Medical Sciences, Yokohama, Japan; Cellular and Molecular Epigenetics Laboratory, Graduate School of Medical Life Science, Yokohama City University, Yokohama, Japan.
Institute of Clinical Chemistry and Laboratory Medicine, University of Regensburg, Regensburg, Germany.
Department of Biochemistry, YLL School of Medicine, National University of Singapore, Singapore.
Department of Laboratory Medicine, National University Hospital, Singapore.
Baker Heart and Diabetes Institute, Melbourne, Victoria, Australia.
Departments of Pharmacology and Medicine, School of Medicine, University of California at San Diego, La Jolla, CA, USA.
Max Planck Institute of Molecular Cell Biology and Genetics, Dresden, Germany.
Department of Biochemistry, YLL School of Medicine, National University of Singapore, Singapore.
Babraham Institute, Cambridge, United Kingdom.
Division of Physiological Chemistry 2, Department of Medical Biochemistry and Biophysics, Karolinska Institute, Stockholm, Sweden.
Singapore Lipidomics Incubator (SLING), Life Sciences Institute, National University of Singapore, Singapore; Department of Biochemistry, YLL School of Medicine, National University of Singapore, Singapore.
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2018 (English)In: Journal of Lipid Research, ISSN 0022-2275, E-ISSN 1539-7262, Vol. 59, no 10, p. 2001-2017Article in journal (Refereed) Published
Abstract [en]
Human blood is a self-regenerating lipid-rich biological fluid that is routinely collected in hospital settings. The inventory of lipid molecules found in blood plasma (plasma lipidome) offers insights into individual metabolism and physiology in health and disease. Disturbances in the plasma lipidome also occur in conditions that are not directly linked to lipid metabolism; therefore, plasma lipidomics based on MS is an emerging tool in an array of clinical diagnostics and disease management. However, challenges exist in the translation of such lipidomic data to clinical applications. These relate to the reproducibility, accuracy, and precision of lipid quantitation, study design, sample handling, and data sharing. This position paper emerged from a workshop that initiated a community-led process to elaborate and define a set of generally accepted guidelines for quantitative MS-based lipidomics of blood plasma or serum, with harmonization of data acquired on different instrumentation platforms across independent laboratories as an ultimate goal. We hope that other fields may benefit from and follow such a precedent.
Place, publisher, year, edition, pages
American Society for Biochemistry and Molecular Biology, 2018. Vol. 59, no 10, p. 2001-2017
Keywords [en]
National Institute of Standards and Technology Standard Reference Material 1950, absolute concentrations, clinical research, clinical trials, data sharing, diagnostic tools, lipids, mass spectrometry, quality control
National Category
Biomedical Laboratory Science/Technology
Identifiers
URN: urn:nbn:se:oru:diva-69218DOI: 10.1194/jlr.S087163ISI: 000445444500018PubMedID: 30115755Scopus ID: 2-s2.0-85054072331OAI: oai:DiVA.org:oru-69218DiVA, id: diva2:1253182
Funder
Swedish Heart Lung Foundation, 20170734 20170603Swedish Research Council
Note
Funding Agencies:
National University of Singapore via the Life Sciences Institute
National Research Foundation Singapore Grant NRFI2015-05
Deutsche Forschungsgemeinschaft TRR83
LiSyM Consortium - Bundesministerium fur Bildung und Forschung, Germany
Foundation for the National Institutes of Health U54 GM069338 RO1 GM20501-41
Japan Agency for Medical Research and Development Grant JP18gm5910001
National Health and Medical Research Council of Australia
Japan Society for the Promotion of Science 15H05897 15H05898 17H03621
Japan Agency for Medical Research and Development Grants 17gm0910005h0403 17gm1010006h0001
National Bioscience Database Center Togo Project, Japan
Biotechnology and Biological Sciences Research Council Grant BB/P013384/1
Medical Research Council Grant MR/M004821/1
Academy of Finland Grant 292568
European Commission Project LITMUS Grant 777377
National Institute of General Medical Sciences Grant R01 GM105724
2018-10-042018-10-042018-10-10Bibliographically approved