oru.sePublications
Change search
CiteExportLink to record
Permanent link

Direct link
Cite
Citation style
  • apa
  • harvard1
  • ieee
  • modern-language-association-8th-edition
  • vancouver
  • Other style
More styles
Language
  • de-DE
  • en-GB
  • en-US
  • fi-FI
  • nn-NO
  • nn-NB
  • sv-SE
  • Other locale
More languages
Output format
  • html
  • text
  • asciidoc
  • rtf
Discovery of mcr-1-Mediated Colistin Resistance in a Highly Virulent Escherichia coli Lineage
The University of Queensland, Brisbane, Queensland, Australia.
Australian Infectious Diseases Research Centre, The University of Queensland, Brisbane, Queensland, Australia; The University of Queensland, UQ Centre for Clinical Research (UQCCR), Herston, Queensland, Australia; College of Medicine, King Saud bin Abdulaziz University for Health Sciences, Riyadh, Saudi Arabia; World Health Organization Collaborating Centre for Infection Prevention and Control, Riyadh, Saudi Arabia; Gulf Cooperation Council Center for Infection Control, Riyadh, Saudi Arabia; King Abdullah International Medical Research Centre, Riyadh, Saudi Arabia.
The University of Queensland, UQ Centre for Clinical Research (UQCCR), Herston, Queensland, Australia; Pathology Queensland, Brisbane, Queensland, Australia.
The University of Queensland, Brisbane, Queensland, Australia.
Show others and affiliations
2018 (English)In: mSphere, ISSN 2379-5042, Vol. 3, no 5, article id e00486-18Article in journal (Refereed) Published
Abstract [en]

Resistance to last-line polymyxins mediated by the plasmid-borne mobile colistin resistance gene (mcr-1) represents a new threat to global human health. Here we present the complete genome sequence of an mcr-1-positive multidrug-resistant Escherichia coli strain (MS8345). We show that MS8345 belongs to serotype O2:K1:H4, has a large 241,164-bp IncHI2 plasmid that carries 15 other antibiotic resistance genes (including the extended-spectrum β-lactamase blaCTX-M-1) and 3 putative multidrug efflux systems, and contains 14 chromosomally encoded antibiotic resistance genes. MS8345 also carries a large ColV-like virulence plasmid that has been associated with E. coli bacteremia. Whole-genome phylogeny revealed that MS8345 clusters within a discrete clade in the sequence type 95 (ST95) lineage, and MS8345 is very closely related to the highly virulent O45:K1:H4 clone associated with neonatal meningitis. Overall, the acquisition of a plasmid carrying resistance to colistin and multiple other antibiotics in this virulent E. coli lineage is concerning and might herald an era where the empirical treatment of ST95 infections becomes increasingly more difficult.

Importance: Escherichia coli ST95 is a globally disseminated clone frequently associated with bloodstream infections and neonatal meningitis. However, the ST95 lineage is defined by low levels of drug resistance amongst clinical isolates, which normally provides for uncomplicated treatment options. Here, we provide the first detailed genomic analysis of an E. coli ST95 isolate that has both high virulence potential and resistance to multiple antibiotics. Using the genome, we predicted its virulence and antibiotic resistance mechanisms, which include resistance to last-line antibiotics mediated by the plasmid-borne mcr-1 gene. Finding an ST95 isolate resistant to nearly all antibiotics that also has a high virulence potential is of major clinical importance and underscores the need to monitor new and emerging trends in antibiotic resistance development in this important global lineage.

Place, publisher, year, edition, pages
American Society for Microbiology , 2018. Vol. 3, no 5, article id e00486-18
Keywords [en]
Escherichia coli, antibiotic resistance, genome analysis
National Category
Microbiology in the medical area Genetics Infectious Medicine
Identifiers
URN: urn:nbn:se:oru:diva-69582DOI: 10.1128/mSphere.00486-18ISI: 000449522900046PubMedID: 30305321Scopus ID: 2-s2.0-85054779630OAI: oai:DiVA.org:oru-69582DiVA, id: diva2:1256314
Note

Funding Agencies:

Royal Brisbane and Women's Hospital Foundation  

National Health and Medical Research Council (NHMRC) of Australia  

NHMRC  

SpeeDx Pty Ltd.  

Merck  

GlaxoSmithKline  

Achaogen  

Superbug Slayers Polo Initiative  

SuperbugsFree movement  

Queensland Polo Association  

Alsace Polo 

Available from: 2018-10-16 Created: 2018-10-16 Last updated: 2018-11-23Bibliographically approved

Open Access in DiVA

No full text in DiVA

Other links

Publisher's full textPubMedScopus

Authority records BETA

Ahmed, Mazen Sid

Search in DiVA

By author/editor
Ahmed, Mazen Sid
By organisation
School of Science and Technology
Microbiology in the medical areaGeneticsInfectious Medicine

Search outside of DiVA

GoogleGoogle Scholar

doi
pubmed
urn-nbn

Altmetric score

doi
pubmed
urn-nbn
Total: 80 hits
CiteExportLink to record
Permanent link

Direct link
Cite
Citation style
  • apa
  • harvard1
  • ieee
  • modern-language-association-8th-edition
  • vancouver
  • Other style
More styles
Language
  • de-DE
  • en-GB
  • en-US
  • fi-FI
  • nn-NO
  • nn-NB
  • sv-SE
  • Other locale
More languages
Output format
  • html
  • text
  • asciidoc
  • rtf