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UV regulates expression of phenylpropanoid biosynthesis genes in cucumber (Cucumis sativus L.) in an organ and spectrum dependent manner
Örebro University, School of Science and Technology. Department of Horticulture, The State Agricultural Ministry Key Laboratory of Horticultural Plant Growth, Development & Quality Improvement, Zhejiang University, Hangzhou, Zhejiang Province, China. (Biokemi)
Örebro University, School of Science and Technology. (Biokemi)
Section of Crop Sciences, Department of Plant and Environmental Sciences, Copenhagen University, University of Copenhagen, Copenhagen, Denmark.
School of Biological, Earth and Environmental Sciences, University College Cork, Cork, Ireland.
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2019 (English)In: Photochemical and Photobiological Sciences, ISSN 1474-905X, E-ISSN 1474-9092, Vol. 18, no 2, p. 424-433Article in journal (Refereed) Published
Abstract [en]

Expression of cucumber (Cucumis sativus) genes encoding the phenylpropanoid and flavonoid biosynthetic enzymes phenylalanine ammonia lyase (PAL), cinnamic acid 4-hydroxylase (C4H), and chalcone synthase (CHS), was studied under control light conditions (photosynthetically active radiation, PAR) in root, stem, and leaf. Furthermore, expression was quantified in leaves illuminated with PAR and supplemental ultraviolet-A (315-400nm) or ultraviolet-B (280-315 nm) radiation. The expression pattern of all twelve CsPAL, threeCsC4H, and three CsCHS genes was established. Among the genes regulated by UV two general expression patterns emerge. One pattern applies to genes primarily regulated by enriched UV-A illumination (pattern 1). Another (pattern 2) was found for the genes regulated by enriched UV-B. Three of the pattern 2 genes (CsPAL4, CsPAL10, CsCHS2) displayed a particular sub-pattern (pattern 2b) with transcription enriched by at least 30 fold. In contrast to the other genes studied, the promoters of the genes regulated according to pattern 2b contained a combination of a number of cis-acting regulatory elements (MREs, ACEs, and G-boxes) that may be of importance for the particularly high enhancement of expression under UV-B- containing light. The regulation of phenylpropanoid and flavonoid biosynthesis genes in cucumber resembles that of a number of other plants. However, cucumber, due to its greater size, is an attractive species for more detailed studies of the fine regulation of spatial and temporal expression of key genes. This in turn, can facilitate the quantitative investigation of the relationships between different promotor motifs, the expression levels of each of these three genes, and metabolite accumulation profiles.

Place, publisher, year, edition, pages
London, UK: Royal Society of Chemistry, 2019. Vol. 18, no 2, p. 424-433
National Category
Biochemistry and Molecular Biology Botany
Research subject
Biochemistry
Identifiers
URN: urn:nbn:se:oru:diva-70117DOI: 10.1039/C8PP00480CISI: 000458569100015PubMedID: 30628617Scopus ID: 2-s2.0-85061384256OAI: oai:DiVA.org:oru-70117DiVA, id: diva2:1262316
Funder
Swedish Research Council Formas, 942-2015-516Knowledge Foundation, 20130164The Swedish Foundation for International Cooperation in Research and Higher Education (STINT), CH2016-6788
Note

Funding Agencies:

Orebro University's Faculty for Business, Science and Technology  

Science Foundation Ireland  SFI 16-IA-4418 

National Natural Science Foundation of China  31711530027 

China Scholarship Council (CSC) 

Available from: 2018-11-11 Created: 2018-11-11 Last updated: 2019-06-18Bibliographically approved

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Qian, MinjieKalbina, IrinaStrid, Åke

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