oru.sePublications
Change search
CiteExportLink to record
Permanent link

Direct link
Cite
Citation style
  • apa
  • harvard1
  • ieee
  • modern-language-association-8th-edition
  • vancouver
  • Other style
More styles
Language
  • de-DE
  • en-GB
  • en-US
  • fi-FI
  • nn-NO
  • nn-NB
  • sv-SE
  • Other locale
More languages
Output format
  • html
  • text
  • asciidoc
  • rtf
Ethylene response factors Pp4ERF24 and Pp12ERF96 regulate blue light-induced anthocyanin biosynthesis in 'Red Zaosu' pear fruits by interacting with MYB114
Department of Horticulture, Zhejiang University, Hangzhou, Zhejiang, People's Republic of China; The Key Laboratory of Horticultural Plant Growth, Development and Quality Improvement, The Ministry of Agriculture of China, Hangzhou, Zhejiang, People's Republic of China; Zhejiang Provincial Key Laboratory of Integrative Biology of Horticultural Plants, Hangzhou, Zhejiang, People's Republic of China.
Department of Horticulture, Zhejiang University, Hangzhou, Zhejiang, People's Republic of China; The Key Laboratory of Horticultural Plant Growth, Development and Quality Improvement, The Ministry of Agriculture of China, Hangzhou, Zhejiang, People's Republic of China; Zhejiang Provincial Key Laboratory of Integrative Biology of Horticultural Plants, Hangzhou, Zhejiang, People's Republic of China.
Department of Horticulture, Zhejiang University, Hangzhou, Zhejiang, People's Republic of China; The Key Laboratory of Horticultural Plant Growth, Development and Quality Improvement, The Ministry of Agriculture of China, Hangzhou, Zhejiang, People's Republic of China; Zhejiang Provincial Key Laboratory of Integrative Biology of Horticultural Plants, Hangzhou, Zhejiang, People's Republic of China.
Örebro University, School of Science and Technology.
Show others and affiliations
2019 (English)In: Plant Molecular Biology, ISSN 0167-4412, E-ISSN 1573-5028, Vol. 99, no 1-2, p. 67-78Article in journal (Refereed) Published
Abstract [en]

KEY MESSAGE: Pp4ERF24 and Pp12ERF96 fine tune blue light-induced anthocyanin biosynthesis via interacting with PpMYB114 and promoting the interaction between PpMYB114 and PpbHLH3, which enhances the expression of PpMYB114-induced PpUFGT.

The red coloration of pear fruit is attributed to anthocyanin accumulation, which is transcriptionally regulated by the MYB-bHLH-WD40 complex. A number of ethylene response factors (ERF) have been identified to regulate anthocyanin biosynthesis in different plants. In pear, several ERF transcription factor genes were identified to be potentially involved in the light-induced anthocyanin biosynthesis according to transcriptome data. But the molecular mechanism of these ERFs underlying the regulation of anthocyanin accumulation is unknown. In this study, exposure of 'Red Zaosu' pear, a mutant of 'Zaosu' pear, to blue light significantly induced the anthocyanin accumulation by increasing the expression levels of anthocyanin biosynthetic genes. Gene expression analysis confirmed that the expression of Pp4ERF24 and Pp12ERF96 genes were up-regulated in the process of blue light-induced anthocyanin biosynthesis. Yeast two-hybrid and bimolecular fluorescence complementation assay revealed that Pp4ERF24 and Pp12ERF96 interacted with PpMYB114, but not with PpMYB10. Bimolecular fluorescence complementation assay demonstrated that the interaction between these two ERFs and PpMYB114 enhanced the interaction between PpMYB114 and PpbHLH3. Further analysis by dual luciferase assay verified that these two ERFs increased the up-regulation of PpMYB114-mediated PpUFGT expression. Furthermore, co-transformation of Pp12ERF96 with PpMYB114 and PpbHLH3 in tobacco leaves led to enhanced anthocyanin accumulation. Transient overexpression of Pp4ERF24 or Pp12ERF96 alone in 'Red Zaosu' pear fruit also induced anthocyanin biosynthesis in pear peel. Our findings provide insights into a mechanism involving the synergistic interaction of ERFs with PpMYB114 to regulate light-dependent coloration and anthocyanin biosynthesis in pear fruits.

Place, publisher, year, edition, pages
Springer, 2019. Vol. 99, no 1-2, p. 67-78
Keywords [en]
Anthocyanin, Blue light, ERF, MYB, Pear (Pyrus)
National Category
Chemical Sciences Biochemistry and Molecular Biology
Identifiers
URN: urn:nbn:se:oru:diva-70807DOI: 10.1007/s11103-018-0802-1ISI: 000457944100005PubMedID: 30539403Scopus ID: 2-s2.0-85058304743OAI: oai:DiVA.org:oru-70807DiVA, id: diva2:1271907
Note

Funding Agencies:

National Natural Science Foundation of China  31471852  31772272 

Earmarked Fund for China Agriculture Research System  CARS-28 

Available from: 2018-12-18 Created: 2018-12-18 Last updated: 2019-02-19Bibliographically approved

Open Access in DiVA

No full text in DiVA

Other links

Publisher's full textPubMedScopus

Authority records BETA

Qian, Minjie

Search in DiVA

By author/editor
Qian, Minjie
By organisation
School of Science and Technology
In the same journal
Plant Molecular Biology
Chemical SciencesBiochemistry and Molecular Biology

Search outside of DiVA

GoogleGoogle Scholar

doi
pubmed
urn-nbn

Altmetric score

doi
pubmed
urn-nbn
Total: 72 hits
CiteExportLink to record
Permanent link

Direct link
Cite
Citation style
  • apa
  • harvard1
  • ieee
  • modern-language-association-8th-edition
  • vancouver
  • Other style
More styles
Language
  • de-DE
  • en-GB
  • en-US
  • fi-FI
  • nn-NO
  • nn-NB
  • sv-SE
  • Other locale
More languages
Output format
  • html
  • text
  • asciidoc
  • rtf