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Effect of PFOS and HBCD on the lipid profiles of developing rainbow trout (Onchorhynchus mykiss) analyzed with UHPLC/Q-TOF-MS
Örebro University, School of Science and Technology.
2018 (English)Independent thesis Basic level (degree of Bachelor), 10 credits / 15 HE creditsStudent thesis
Abstract [en]

Perfluorooctane sulfonate (PFOS) is widely used in industrial products and is potentially dangerous to the aquatic environment due to not being broken down whether by chemical or biological means, having a half-life of more than 41 years and disrupting hormones. Hexabromocyclododecane (HBCD) is the third most used brominated flame retardant and is of environmental concern as it bioaccumulates and magnifies in the food chain and is highly toxic to aquatic organisms. The purpose of this study was to examine the effect of PFOS and HBCD on the embryos of rainbow trout (Onchorhynchus mykiss) by analyzing lipid profiles with UHPLC/Q-TOF-MS. The fish embryos were treated with various concentrations of PFOS and HBCD (0.058-58 μg/l and 0.014-14 μg/l respectively) with DMSO as carrier solvent and then extracted after homogenization with 0.9% NaCl-solution followed by addition of ISTD mixture, methanol, methyl tert-butyl ether (MTBE) and MQ-water. The raw data was processed with MZmine-2.32. 153 lipids were identified with the main lipids consisting of glycerophospholipids and triacylglycerols. A two-tailed t-test was used to study the impact of the chemical exposure on the embryos, where p-values below 0.05 were lipids considered as significant change. The HBCD exposure caused significant change in various triacylglycerols, whereas PFOS exposure caused significant change in triacylglycerols as well as in glycerophospholipids such as PC(O-38:5) and LPC(20:4). The results were in alignment with previous studies.

Place, publisher, year, edition, pages
2018. , p. 34
Keywords [en]
Perfluorooctane sulfonate, hexabromocyclododecane, lipid metabolism, UHPLC/Q-TOF-MS
National Category
Chemical Sciences
Identifiers
URN: urn:nbn:se:oru:diva-71044OAI: oai:DiVA.org:oru-71044DiVA, id: diva2:1274051
Subject / course
Chemistry
Supervisors
Examiners
Available from: 2018-12-27 Created: 2018-12-27 Last updated: 2019-03-04Bibliographically approved

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Effect of PFOS and HBCD on the lipid profiles of developing rainbow trout (Onchorhynchus mykiss) analyzed with UHPLC/Q-TOF-MS(660 kB)15 downloads
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CiteExportLink to record
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Citation style
  • apa
  • ieee
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Language
  • de-DE
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  • en-US
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  • nn-NB
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  • Other locale
More languages
Output format
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