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Heat Shock Factor 5 Is Essential for Spermatogenesis in Zebrafish
Reproductive Genomics Group, Temasek Life Sciences Laboratory, Singapore, Singapore.
Reproductive Genomics Group, Temasek Life Sciences Laboratory, Singapore, Singapore; Department of Biological Sciences, National University of Singapore, Singapore, Singapore.
Reproductive Genomics Group, Temasek Life Sciences Laboratory, Singapore, Singapore.
Örebro University, School of Science and Technology. Biology, Örebro Life Science Center.ORCID iD: 0000-0003-3302-7106
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2018 (English)In: Cell reports, ISSN 2211-1247, E-ISSN 2211-1247, Vol. 25, no 12, p. 3252-3261Article in journal (Refereed) Published
Abstract [en]

Heat shock factors (Hsfs) are transcription factors that regulate responses to heat shock and other environmental stimuli. Four heat shock factors (Hsf1-4) have been characterized from vertebrates to date. In addition to stress response, they also play important roles in development and gametogenesis. Here, we study the fifth member of heat shock factor family, Hsf5, using zebrafish as a model organism. Mutant hsf5(-/-) males, generated by CRISPR/Cas9 technique, were infertile with drastically reduced sperm count, increased sperm head size, and abnormal tail architecture, whereas females remained fertile. We show that Hsf5 is required for progression through meiotic prophase 1 during spermatogenesis as suggested by the accumulation of cells in the leptotene and zygotene-pachytene stages and increased apoptosis in post-meiotic cells. hsf5(-/-) mutants show gonadal misregulation of a substantial number of genes with roles in cell cycle, apoptosis, protein modifications, and signal transduction, indicating an important role of Hsf5 in early stages of spermatogenesis.

Place, publisher, year, edition, pages
Cell Press , 2018. Vol. 25, no 12, p. 3252-3261
National Category
Cell Biology
Identifiers
URN: urn:nbn:se:oru:diva-71238DOI: 10.1016/j.celrep.2018.11.090ISI: 000453826600003PubMedID: 30566854Scopus ID: 2-s2.0-85058181011OAI: oai:DiVA.org:oru-71238DiVA, id: diva2:1276899
Funder
Knowledge Foundation
Note

Funding Agencies:

National Research Foundation, Prime Minister's Office, Singapore under its Competitive Research Programme  NRF-CRP7-2010-001 

National Research, Development and Innovation Office of Hungary through its Frontline Research Grant  KKP 126764 

Temasek Life Sciences Laboratory  

Örebro University 

Available from: 2019-01-09 Created: 2019-01-09 Last updated: 2019-01-09Bibliographically approved

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