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Flow Cytometry Assay for Recycling of LFA-1 in T-lymphocytes
Department of Experimental Medical Science, Lund University, Lund, Sweden.
Department of Experimental Medical Science, Lund University, Lund, Sweden.
Department of Experimental Medical Science, Lund University, Lund, Sweden.
Örebro University, School of Medical Sciences. Department of Experimental Medical Science, Lund University, Lund, Sweden.
2018 (English)In: Bio-protocol, E-ISSN 2331-8325, Vol. 8, no 23, article id e3104Article in journal (Refereed) Published
Abstract [en]

To enable cells to move forward, cell surface integrins are internalized into an endosomal compartment and subsequently intracellularly transported to be re-exposed at a new site on the cell membrane. Leukocytes are the fastest migrating cell type in the human body, which express the leukocyte-specific integrin LFA-1. Here, we describe a flow cytometry-based assay that allows the quantification of LFA-1 internalization and its re-expression on the cell surface in T lymphocytes. An advantage of using flow cytometry-based assay over biochemical methods is the low number of needed cells. This protocol can be also used to measure recycling of other receptors.

Place, publisher, year, edition, pages
Bio-Protocol , 2018. Vol. 8, no 23, article id e3104
Keywords [en]
Flow cytometry, Receptor, LFA-1, Endocytosis, Exocytosis
National Category
Medical Biotechnology (with a focus on Cell Biology (including Stem Cell Biology), Molecular Biology, Microbiology, Biochemistry or Biopharmacy)
Identifiers
URN: urn:nbn:se:oru:diva-72537DOI: 10.21769/BioProtoc.3104ISI: 000458028500009OAI: oai:DiVA.org:oru-72537DiVA, id: diva2:1289838
Funder
Swedish Research Council, K2010-80P-21592-01-4 K2010-80X-215917-01-4The Royal Swedish Academy of SciencesÅke Wiberg Foundation
Note

Funding Agencies:

Foundation Olle Engquist Byggmästare  

I&A Lundberg Research Foundation  

Royal Physiographic Society of Lund  

Jeanssons Foundation  

Kocks Foundation  

P & U Schybergs Foundation  

Gyllenstiernska Krapperup Foundation  

Gustav V 80 Jubilee Fund  

Österlund Foundation  

Nanna Svartz and Crafoord awards  

Anna-Greta Crafoord postdoctoral fellowship

Available from: 2019-02-19 Created: 2019-02-19 Last updated: 2019-02-19Bibliographically approved

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Svensson, Lena

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