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Regulation of Arabidopsis gene expression by low fluence rate UV-B independently of UVR8 and stress signaling
Örebro University, School of Science and Technology. Institute of Molecular, Cell and Systems Biology, College of Medical, Veterinary and Life Sciences, University of Glasgow, Glasgow, UK. (Molecular Biochemistry, The Life Science Centre)ORCID iD: 0000-0002-8834-4064
Institute of Molecular, Cell and Systems Biology, College of Medical, Veterinary and Life Sciences, University of Glasgow, Glasgow, UK.
Institute of Molecular, Cell and Systems Biology, College of Medical, Veterinary and Life Sciences, University of Glasgow, Glasgow, UK.
Örebro University, School of Science and Technology. (Molecular Biochemistry, The Life Science Centre)ORCID iD: 0000-0002-9233-7254
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2019 (English)In: Photochemical and Photobiological Sciences, ISSN 1474-905X, E-ISSN 1474-9092, Vol. 18, no 7, p. 1675-1684Article in journal (Refereed) Published
Abstract [en]

UV-B exposure of plants regulates expression of numerous genes concerned with various responses. Sudden exposure of non-acclimated plants to high fluence rate, short wavelength UV-B induces expression via stress-related signaling pathways that are not specific to the UV-B stimulus, whereas low fluence rates of UV-B can regulate expression via the UV-B photoreceptor UV RESISTANCE LOCUS 8 (UVR8). However, there is little information about whether non-stressful, low fluence rate UV-B treatments can activate gene expression independently of UVR8. Here, transcriptomic analysis of wild-type and uvr8 mutant Arabidopsis exposed to low fluence rate UV-B showed that numerous genes were regulated independently of UVR8. Moreover, nearly all of these genes were distinct to those induced by stress treatments. A small number of genes were expressed at all UV-B fluence rates employed and may be concerned with activation of eustress responses that facilitate acclimation to changing conditions. Expression of the gene encoding the transcription factor ARABIDOPSIS NAC DOMAIN CONTAINING PROTEIN 13 (ANAC13) was studied to characterise a low fluence rate, UVR8-independent response. ANAC13 is induced by as little as 0.1 μmol m−2 s−1 UV-B and its regulation is independent of components of the canonical UVR8 signaling pathway COP1 and HY5/HYH. Furthermore, UV-B induced expression of ANAC13 is independent of the photoreceptors CRY1, CRY2, PHOT1 and PHOT2 and phytochromes A, B, D and E. ANAC13 expression is induced over a range of UV-B wavelengths at low doses, with maximum response at 310 nm. This study provides a basis for further investigation of UVR8 and stress independent, low fluence rate UV-B signaling pathway(s).

Place, publisher, year, edition, pages
RSC Publishing, 2019. Vol. 18, no 7, p. 1675-1684
National Category
Biochemistry and Molecular Biology
Identifiers
URN: urn:nbn:se:oru:diva-74760DOI: 10.1039/C9PP00151DISI: 000477947100005PubMedID: 31218318Scopus ID: 2-s2.0-85068743324OAI: oai:DiVA.org:oru-74760DiVA, id: diva2:1328135
Funder
Knowledge Foundation, 20130164Swedish Research Council Formas, 942-2015-516
Note

Funding Agencies:

UK Biotechnology and Biological Sciences research council PhD studentships at the University of Glasgow

Faculty for Business, Science, and Technology at Örebro University  

Strategic Young Researchers Recruitment Programme at Örebro University  

University of Glasgow 

Available from: 2019-06-20 Created: 2019-06-20 Last updated: 2019-08-12Bibliographically approved

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O'Hara, AndrewMorales, Luis OrlandoStrid, Åke

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