Per- and polyfluorinated alkyl substances (PFAS) and perfluorooctance sulphonic acid(PFOS) have been detected in human blood and wild animals. There is widespread use ofPFAS in products such as foaming agents, emulsifiers and surface protection treatments andthus humans are in continual contact with these substances through textiles, cookware andcosmetics. PFAS are persistent in the environment and therefore bioaccumulate in organisms.The scope of this study was to analyze the effects of microbial bound PFAS on lifespan andfertility of the in vivo model C. elegans fed with PFAS-treated Pseudomonas nitroreducens,Acidovorax delafieldii and Escherichia coli. There was no significant difference in the C.elegans lifespan when fed E. coli with K-medium, environmental waters and a PFAS mixtureand no worms fed with the other two bacteria survived. The worms survived in all conditionsfor the egg production and hatching, but no statistical difference was found. Further researchis needed to investigate the effects of microbial bound PFAS in C. elegans and the impact ofdifferent bacteria as food.