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Effect of lidocaine and ropivacaine on primary (SW480) and metastatic (SW620) colon cancer cell lines
Örebro University, School of Medical Sciences. Department of Anesthesiology and Intensive Care.
Örebro University, School of Medical Sciences. Department of Clinical Research Laboratory,.
Örebro University, School of Medical Sciences. Department of Clinical Research Laboratory.ORCID iD: 0000-0001-8304-2772
Department of Physiology and Pharmacology, Karolinska Institute and Karolinska University Hospital, Stockholm; School of Medical Sciences, Örebro University, Örebro, Sweden.
2019 (English)In: Oncology Letters, ISSN 1792-1074, E-ISSN 1792-1082, Vol. 18, no 1, p. 395-401Article in journal (Refereed) Published
Abstract [en]

Regional anesthesia may prolong survival following surgery for different types of cancers. The mechanisms behind this are unclear but direct effects on cancer cells by local anesthetics (LA) have been suggested. The aim of this study was to investigate if lidocaine or ropivacaine have a dose-dependent effect on the cell viability and proliferation of a primary and a secondary colon carcinoma cell line in vitro. The colon cancer cell lines SW480 derived from primary tumor and SW620 from a metastatic site in the same patient were exposed to increasing concentrations of lidocaine and ropivacaine (5-1,000 mu M). Cell viability was measured using CellTiter-Blue((R)) and cell proliferation by PKH67 after exposure for up to 72 h. Cell viability was significantly reduced by ropivacaine at the highest concentration (1,000 mu M) after 48 and 72 h in the cell line SW480 and at 72 h in SW620. Exposure to lidocaine did not show any significant reduction in cell viability. Notably, low concentrations of both lidocaine and ropivacaine significantly increased cell viability after 48 and 72 h in SW620. Cell proliferation was significantly reduced by 1,000 mu M lidocaine in SW480 and by 1,000 mu M ropivacaine in SW620. In summary, both lidocaine and ropivacaine showed an anti-proliferative effect in the colon cancer cell lines at high concentrations and after prolonged exposure to LA in vitro. Our findings also indicate that lower concentrations promote cell viability in the metastatic cell line.

Place, publisher, year, edition, pages
Spandidos Publications , 2019. Vol. 18, no 1, p. 395-401
Keywords [en]
local anesthetics, colon cancer, SW480, SW620, in vitro, cell viability, proliferation
National Category
Cancer and Oncology
Identifiers
URN: urn:nbn:se:oru:diva-75408DOI: 10.3892/ol.2019.10332ISI: 000474896900047Scopus ID: 2-s2.0-85068799738OAI: oai:DiVA.org:oru-75408DiVA, id: diva2:1339562
Note

Funding Agency:

Research Committee of the Örebro County Council

Available from: 2019-07-30 Created: 2019-07-30 Last updated: 2019-07-30Bibliographically approved

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Siekmann, WiebkeTina, ElisabetKoskela von Sydow, Anita

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