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Benzo[a]pyrene and 2,3-benzofuran induce divergent temporal patterns of AhR-regulated responses in zebrafish embryos (Danio rerio)
Department of Ecosystem Analysis, Institute for Environmental Research, ABBt - Aachen Biology and Biotechnology, RWTH Aachen University, Aachen, Germany.
Örebro University, School of Science and Technology. (Man-Technology-Environment Research Centre)
Department of Ecosystem Analysis, Institute for Environmental Research, ABBt - Aachen Biology and Biotechnology, RWTH Aachen University, Aachen, Germany.
Örebro University, School of Science and Technology. (Man-Technology-Environment Research Centre)ORCID iD: 0000-0002-2356-6686
2019 (English)In: Ecotoxicology and Environmental Safety, ISSN 0147-6513, E-ISSN 1090-2414, Vol. 183, article id 109505Article in journal (Refereed) Epub ahead of print
Abstract [en]

Biotests like the fish embryo toxicity test have become increasingly popular in risk assessment and evaluation of chemicals found in the environment. The large range of possible endpoints is a big advantage when researching on the mode of action of a certain substance. Here, we utilized the frequently used model organism zebrafish (Danio rerio) to examine regulative mechanisms in the pathway of the aryl-hydrocarbon receptor (AHR) in early development. We exposed embryos to representatives of two chemical classes known to elicit dioxin-like activity: benzo[a]pyrene for polycyclic aromatic hydrocarbons (PAHs) and 2,3-benzofuran for polar O-substituted heterocycles as a member of heterocyclic compounds in general (N-, S-, O-heterocycles; NSO-hets). We measured gene transcription of the induced P450 cytochromes (cyp1), their formation of protein and biotransformation activity throughout the whole embryonic development until 5 days after fertilization. The results show a very specific time course of transcription depending on the chemical properties (e.g. halogenation, planarity, Kow), the physical decay and the biodegradability of the tested compound. However, although this temporal pattern was not precisely transferable onto the protein level, significant regulation in enzymatic activity over time could be detected. We conclude, that a careful choice of time and end point as well as consideration of the chemical properties of a substance are fairly important when planning, conducting and especially evaluating biotests.

Place, publisher, year, edition, pages
Academia Press, 2019. Vol. 183, article id 109505
Keywords [en]
Aryl hydrocarbon receptor, Biotransformation, Cytochrome P450, Dioxin-like activity, EROD, Zebrafish embryo
National Category
Pharmacology and Toxicology
Identifiers
URN: urn:nbn:se:oru:diva-75813DOI: 10.1016/j.ecoenv.2019.109505PubMedID: 31394372OAI: oai:DiVA.org:oru-75813DiVA, id: diva2:1345137
Available from: 2019-08-23 Created: 2019-08-23 Last updated: 2019-08-23Bibliographically approved

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Larsson, MariaKeiter, Steffen

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