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Metabolomic changes in fatty liver can be modified by dietary protein and calcium during energy restriction
Institute of Biomedicine, Pharmacology, Biomedicum Helsinki, University of Helsinki, Helsinki, Finland; Foundation for Nutrition Research, Helsinki, Finland; Valio Ltd, Research Center, Valio, Helsinki, Finland.
VTT Technical Research Centre of Finland, Finland.
VTT Technical Research Centre of Finland, Finland.
Institute of Biomedicine, Pharmacology, Biomedicum Helsink, University of Helsinki, Helsinki, Finland.
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2008 (English)In: World Journal of Gastroenterology, ISSN 1007-9327, E-ISSN 2219-2840, Vol. 14, no 28, p. 4462-4472Article in journal (Refereed) Published
Abstract [en]

AIM: To characterise the effect of energy restriction (ER) on liver lipid and primary metabolite profile by using metabolomic approach. We also investigated whether the effect of energy restriction can be further enhanced by modification of dietary protein source and calcium.

METHODS: Liver metabolomic profile of lean and obese C57Bl/6J mice (n = 10/group) were compared with two groups of weight-reduced mice. ER was performed on control diet and whey protein-based high-calcium diet (whey + Ca). The metabolomic analyses were performed using the UPLC/MS based lipidomic platform and the HPLC/MS/MS based primary metabolite platform.

RESULTS: ER on both diets significantly reduced hepatic lipid accumulation and lipid droplet size, while only whey + Ca diet significantly decreased blood glucose (P < 0.001) and serum insulin (P < 0.01). In hepatic lipid species the biggest reduction was in the level of triacylglycerols and ceramides while the level of cholesterol esters was significantly increased during ER. Interestingly, diacylglycerol to phospholipid ratio, an indicator of relative amount of diabetogenic diglyceride species, was increased in the control ER group, but decreased in the whey + Ca ER group (P < 0.001, vs obese). ER on whey + Ca diet also totally reversed the obesity induced increase in the relative level of lipotoxic ceramides (P < 0.001, vs obese; P > 0.05, vs lean). These changes were accompanied with up-regulated TCA cycle and pentose phosphate pathway metabolites.

CONCLUSION: ER-induced changes on hepatic metabolomic profile can be significantly affected by dietary protein source. The therapeutic potential of whey protein and calcium should be further studied.

Place, publisher, year, edition, pages
Baishideng Publishing Group Co., Limited , 2008. Vol. 14, no 28, p. 4462-4472
Keywords [en]
Fatty liver, metabolomics, energy restriction, whey protein, dietary calcium
National Category
Nutrition and Dietetics
Identifiers
URN: urn:nbn:se:oru:diva-70920DOI: 10.3748/wjg.14.4462ISI: 000258263500006PubMedID: 18680224Scopus ID: 2-s2.0-58049108115OAI: oai:DiVA.org:oru-70920DiVA, id: diva2:1345906
Available from: 2019-08-26 Created: 2019-08-26 Last updated: 2019-08-28Bibliographically approved

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