Application value of CyTOF 2 mass cytometer technology at single-cell level in human gastric cancer cellsShow others and affiliations
2019 (English)In: Experimental Cell Research, ISSN 0014-4827, E-ISSN 1090-2422, Vol. 384, no 1, article id 111568Article in journal (Refereed) Published
Abstract [en]
Chemotherapy and radiotherapy are main adjuvant therapies for the treatment of gastric cancer, the treatment effects are individual difference, but the specific mechanism is unknown. CyTOF 2 mass cytometer (CyTOF) enables the detecting up to 135 parameters on single cell, the emergence of which is an opportunity for proteomics research. We first tried to apply CyTOF technique to gastric cancer cells. We verified applicability of CyTOF in gastric cancer cells, and analyzed the responses of seventeen proteins to chemoradiotherapy in human gastric cancer AGS cells. To analyze the high dimensional CyTOF data, we used two statistical and visualization tools including viSNE and Citrus. Two specific clusters were found which had differences in protein expression profiles. CyTOF technology is proved feasibility and value at single cell level of gastric cancer.
Place, publisher, year, edition, pages
Elsevier, 2019. Vol. 384, no 1, article id 111568
Keywords [en]
Citrus, Gastric cancer, Mass cytometry, Single-cell, viSNE
National Category
Cancer and Oncology
Identifiers
URN: urn:nbn:se:oru:diva-75898DOI: 10.1016/j.yexcr.2019.111568ISI: 000490031700024PubMedID: 31446162Scopus ID: 2-s2.0-85072229049OAI: oai:DiVA.org:oru-75898DiVA, id: diva2:1349496
Note
Funding Agencies:
Hebei Province Key Research and Development Project 18277741D
International Science & Technology Cooperation Program of China 2014DFA31150
Hebei Province Projects 1387 SGH201501 A201802017 LNB201809 G2019035
2019-09-092019-09-092024-03-05Bibliographically approved