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Multi-Laboratory Hazard Assessment of Contaminated Microplastic Particles by Means of Enhanced Fish Embryo Test With the Zebrafish (Danio rerio)
Örebro University, School of Science and Technology. University of Bordeaux, EPOC UMR CNRS 5805, Pessac, France. (Man-Technology-Environment Research Centre (MTM))
Aquatic Ecology and Toxicology Group, Center for Organismal Studies, University of Heidelberg, Heidelberg, Germany.
University of Bordeaux, EPOC UMR CNRS 5805, Pessac, France.
Laboratoire Ressources Halieutiques, IFREMER, L’Houmeau, France.
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2019 (English)In: Frontiers in Environmental Science, E-ISSN 2296-665X, Vol. 7, article id 135Article in journal (Refereed) Published
Abstract [en]

As wide-spread pollutants in the marine environment, microplastics (MPs) have raised public concern about potential toxic effects in aquatic organisms, and, among others, MPs were suspected to act as a vector for organic pollutants to biota. The purpose of the present study was to investigate effects by three model pollutants, oxybenzone (BP3), benzo[a] pyrene (BaP), and perfluorooctane sulfonate (PFOS) adsorbed to polyethylene MPs on the basis of a standard assay, the acute fish embryo toxicity test (FET; OECD TG 236) with zebrafish (Danio rerio) supplemented by additional endpoints such as induction of ethoxyresorufin-O-deethylase (EROD) activity, modification of cyp1a gene transcription and changes in larval swimming behavior. FET assays were performed in three laboratories using slightly different husbandry and exposure conditions, which, however, were all fully compatible with the limits defined by OECD TG 236. This allowed for testing of potential changes in the FET assay due to protocol variations. The standard endpoints of the FET (acute embryotoxicity) did not reveal any acute toxicity for both virgin MPs and MPs spiked with BP3, BaP, and PFOS. With respect to sublethal endpoints, EROD activity was increased after exposure to MPs spiked with BP3 (3 h pulse) and MPs spiked with BaP (96 h continuous exposure). Cyp1a transcription was increased upon exposure to MPs spiked with BP3 or BaP. For the selected combination of MPs particles and contaminants, the basic FET proved not sensitive enough to reveal effects of (virgin and spiked) MPs. However, given that the FET can easily be supplemented by a broad variety of more subtle and sensitive endpoints, an enhanced FET protocol may provide a relevant approach with developmental stages of a vertebrate animal model, which is not protected by current EU animal welfare legislation (Directive EU 2010/63).

Place, publisher, year, edition, pages
Frontiers Media S.A., 2019. Vol. 7, article id 135
Keywords [en]
fish embryotoxicity test (FET), swimming behavior, EROD, cyp1a, perfluorooctane sulfonate, benzo[a]pyrene, oxybenzone
National Category
Environmental Sciences
Identifiers
URN: urn:nbn:se:oru:diva-76995DOI: 10.3389/fenvs.2019.00135ISI: 000486181200001Scopus ID: 2-s2.0-85072891874OAI: oai:DiVA.org:oru-76995DiVA, id: diva2:1357185
Note

Funding Agencies:

JPI Oceans  FCT JPIOCEANS/0005/2015 ANR-15-JOCE-0002-01 BMBF 03F0735A

University of Bordeaux  

German Academic Scholarship Foundation (Studienstiftung des Deutschen Volkes)  

JPI Oceans (FORMAS)  2015-01865

Available from: 2019-10-03 Created: 2019-10-03 Last updated: 2023-12-08Bibliographically approved

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Cormier, BettieKeiter, Steffen

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