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Human listeriosis: sources and routes
Örebro University, School of Health and Medical Sciences. Örebro University, School of Hospitality, Culinary Arts & Meal Science.
2008 (English)Doctoral thesis, comprehensive summary (Other academic)
Abstract [en]

The bacterium Listeria monocytogenes can cause the disease listeriosis in both humans and animals. For the epidemiological investigation of listeriosis detection and characterisation of the organism are important steps.

Paper I. There are few reports on the incidence of L. monocytogenes in clinical samples from humans in India. Therefore, we investigated 144 samples from immunocompromised patients. L. monocytogenes was isolated from two placental bits from women with poor obstetric history, one patient with renal failure and three other patients. Five isolates were positive for the virulence genes hlyA, actA and iap. The sixth isolate was positive for hlyA and actA genes.

Paper II. Characterisation of 601 human L. monocytogenes isolates causing invasive listeriosis during the period 1986 to 2007 in Sweden reveals a decrease in serovar 4b strains. Since 1996, serovar 1/2a has become the predominant serovar causing human listeriosis: PFGE analysis revealed two clusters including different serotypes suggesting that we need more studies on genetic relatedness among clinical isolates.

Paper III. The incidence of Listeria species in seafood from markets in Goa was studied. One hundred and fifteen raw/fresh seafoods bought at the fish markets were sampled and tested for presence of Listeria spp. L. monocytogenes was detected in 10 samples. L. monocytogenes in raw seafood may pose a health risk in kitchen if contaminating ready-to-eat food.

Paper IV. Gravad and cold-smoked salmon are associated with human listeriosis in Sweden. L. monocytogenes was isolated from 11 of 56 products. Serovar 1/2a was predominant, followed by 4b. REA/PFGE typing of the isolates identified five types of L. monocytogenes. One type was identical to a human type, two other were closely related.These findings suggest that gravad and cold-smoked salmon are still possible sources of listeriosis in Sweden.

Paper V. Many outbreaks of listeriosis have been related to consumption of dairy-associated products. Therefore, 123 farm bulk milk samples in India and 20 cervico-vaginal samples from dairy cows with reproductive disorders were investigated for L. monocytogenes.

L. monocytogenes was isolated from 17.9% of bulk milk samples and from 10% of cervico-vaginal swabs. The virulence gene hlyA was detected in all isolates. These findings represent a public health risk where unpasteurised milk and milk products are largely consumed.

Paper VI. Isolates of L. monocytogenes (n=177) from 22 animal species were characterized and compared with human strains isolated between 1986-2006 in Sweden. Although many animals and humans shared pulsovars, they did not appear at the same time or with the same proportion of strains. The pulsovars shared by both animals and humans may indicate that there is an exchange of L. monocytogenes strains between these two groups due to either direct or indirect transmission.

 

Place, publisher, year, edition, pages
Örebro: Örebro universitet , 2008. , p. 89
Series
Örebro Studies in Medicine, ISSN 1652-4063 ; 19
Keyword [en]
Listeria monocytogenes, listeriosis, zoonoses, PFGE, food safety, incidence
National Category
Medical and Health Sciences
Research subject
Biomedicine
Identifiers
URN: urn:nbn:se:oru:diva-2447ISBN: 978-91-7668-612-6 (print)OAI: oai:DiVA.org:oru-2447DiVA, id: diva2:136263
Public defence
2008-09-29, Gastronomiska teatern, Måltidens hus, Sörälgsvägen 2, Grythyttan, 13:00 (English)
Opponent
Supervisors
Note

The work is done in cooperation with the School of Hospitality, Culinary Arts & Meal Science, Örebro UniversityVishal Singh Parihar, Örebro University, Department of Restaurant and Culinary Arts, Sörälgsvägen 2, SE-712 60 Grythyttan, Sweden or ICAR Research Complex for Goa, Ela, Old Goa – 403402, Goa, India. Phone 0832-2284678/79; Fa:0832-2285649. E-mail: drvishu@yahoo.co.in

Available from: 2008-09-05 Created: 2008-09-05 Last updated: 2017-10-18Bibliographically approved
List of papers
1. Isolation of Listeria monocytogenes from human clinical cases in India
Open this publication in new window or tab >>Isolation of Listeria monocytogenes from human clinical cases in India
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(English)Manuscript (preprint) (Other academic)
National Category
Medical Biotechnology
Research subject
Biomedicine
Identifiers
urn:nbn:se:oru:diva-15595 (URN)
Available from: 2011-05-17 Created: 2011-05-17 Last updated: 2017-10-17Bibliographically approved
2. Characterization of human invasive isolates of Listeria monocytogenes in Sweden 1986-2007
Open this publication in new window or tab >>Characterization of human invasive isolates of Listeria monocytogenes in Sweden 1986-2007
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2008 (English)In: Foodborne pathogens and disease, ISSN 1535-3141, E-ISSN 1556-7125, Vol. 5, no 6, p. 755-761Article in journal (Refereed) Published
Abstract [en]

Since 1986, 68% of the Listeria monocytogenes isolates from human cases of invasive listeriosis in Sweden are available for retrospective studies. The aim of the present study was to characterize 601 human invasive isolates of L. monocytogenes in Sweden from 1986 to 2007 by using serotyping and pulsed-field gel electrophoresis. Since 1996, serovar 4b was permanently reduced to the second or third most common serovar in human cases in Sweden. During the latter period, 2000-2007, only 13% belonged to serovar 4b and 71% to 1/2a. The dendrogram, based on pulsovars, reveals two clusters with different serovars. Cluster 1 exhibits serovars 4b and 1/2b, whereas cluster 2 consists of serovar 1/2a. Serovar 1/2a seems to be more heterogeneous than serovar 4b.

Place, publisher, year, edition, pages
Mary Ann Liebert, 2008
Keyword
Invasive, Listeria monocytogenes, retrospective
National Category
Microbiology in the medical area
Research subject
Microbiology; Culinary Arts and Meal Science
Identifiers
urn:nbn:se:oru:diva-2997 (URN)10.1089/fpd.2008.0123 (DOI)000262028400007 ()18847381 (PubMedID)2-s2.0-57749091480 (Scopus ID)
Note

The work is done in cooperation with the School of Hospitality, Culinary Arts & Meal Science, Örebro University

Available from: 2008-09-05 Created: 2008-09-05 Last updated: 2018-01-13Bibliographically approved
3. Isolation and characterization of Listeria species from tropical seafoods
Open this publication in new window or tab >>Isolation and characterization of Listeria species from tropical seafoods
2008 (English)In: Food Control, ISSN 0956-7135, E-ISSN 1873-7129, Vol. 19, no 6, p. 566-569Article in journal (Refereed) Published
Abstract [en]

The incidence of Listeria species in the seafood of markets in Goa, India was studied. One hundred and fifteen raw/fresh seafoods bought at the fish markets were sampled and tested for presence of Listeria spp. using a two step enrichment procedure, followed by plating on two selective agars. The confirmation of the isolates was based on biochemical identification. Twenty eight seafood samples were positive for Listeria spp. and in 10 samples Listeria monocytogenes was detected. L.innocua was the most common Listeria species recovered and was detected in 18 samples. L. monocytogenes in raw seafood may pose a health risk in kitchen if contaminating ready-to-eat food.

Place, publisher, year, edition, pages
Amsterdam: Elsevier, 2008
National Category
Medical and Health Sciences Microbiology in the medical area Social Sciences
Research subject
Microbiology; Culinary Arts and Meal Science; Biomedicine
Identifiers
urn:nbn:se:oru:diva-2998 (URN)10.1016/j.foodcont.2007.06.009 (DOI)
Note

The work is done in cooperation with the School of Hospitality, Culinary Arts & Meal Science, Örebro University

Available from: 2008-09-05 Created: 2008-09-05 Last updated: 2018-01-13Bibliographically approved
4. Gravad (Gravlax) and cold-smoked salmon, still a potential source of listeriosis
Open this publication in new window or tab >>Gravad (Gravlax) and cold-smoked salmon, still a potential source of listeriosis
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2009 (English)In: Journal of Foodservice, ISSN 1748-0140, Vol. 20, no 1, p. 15-20Article in journal (Refereed) Published
Abstract [en]

Gravad (Gravlax) and cold-smoked salmon are associated with human listeriosis in Sweden. The present investigation of Listeria monocytogenes in salmon was a follow-up of a similar study in the middle of the 1990s. Since our first study, there has been an increasing focus on food hygiene in general and specially on self-inspection and Hazard Analysis and Critical Control Points from the authorities and food producers. L. monocytogenes was isolated from 11 (three manufacturers) of the 56 products analyzed. The highest level of L. monocytogenes was 1500 cfu/g from a cold-smoked salmon product; however, the level was low (<100 cfu/g) in most products. Serovar 1/2a was predominant, followed by 4b. restriction enzyme analysis/ pulsed-field gel electrophoresis typing of the 56 salmon isolates identified five types of L. monocytogenes. One type was identical to a human type, whereas two other were closely related. These findings suggest that gravad and coldsmoked salmon are still possible sources of listeriosis in Sweden.

Place, publisher, year, edition, pages
Oxford: Blackwell Publishing, 2009
National Category
Medical and Health Sciences
Research subject
Culinary Arts and Meal Science; Biomedicine
Identifiers
urn:nbn:se:oru:diva-2999 (URN)10.1111/j.1748-0159.2008.00118.x (DOI)
Available from: 2008-09-05 Created: 2008-09-05 Last updated: 2017-10-18Bibliographically approved
5. Isolation of Listeria species from farm bulk milk at the receiving dairy plant and cervico-vaginal swabs
Open this publication in new window or tab >>Isolation of Listeria species from farm bulk milk at the receiving dairy plant and cervico-vaginal swabs
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2007 (English)In: Indian Journal of Comparative Microbiology, Immunology and Infectious Diseases, ISSN 0974-0147, Vol. 28, no 1/2, p. 53-55Article in journal (Refereed) Published
Abstract [en]

Listeria species were isolated from farm bulk milk (n=123) at the receiving dairy plant and cervico-vaginal samples (n=20) collected from dairy cows with reproductive disorders. Following enrichment and plating on two selective agar media, confirmation of the isolates was based on standard tests. Isolates were subjected to a PCR assay for detection of the hlyA gene. Overall, Listeria spp. were isolated from 30(24.4%) bulk milk samples and four (20%) cervico-vaginal swabs. Listeria monocytogenes was isolated from 17.9%, L. innocua from 1.6%, L. seeligeri from 3.3% and L. welshimeri from 1.6% bulk milk samples. Only L. monocytogenes (10%) and L. innocua (10%) could be isolated from cervico-vaginal swabs. The hlyA gene was detected in all L. monocytogenes isolates. These findings represent a public health risk where homemade unpasteurised milk and milk products are largely consumed.

National Category
Medical and Health Sciences Infectious Medicine
Research subject
Biomedicine
Identifiers
urn:nbn:se:oru:diva-3000 (URN)
Available from: 2008-09-05 Created: 2008-09-05 Last updated: 2017-10-18Bibliographically approved
6. Molecular characterisation of Listeria monocytogenes isolates from animals in Sweden and comparison with previously characterised human strains
Open this publication in new window or tab >>Molecular characterisation of Listeria monocytogenes isolates from animals in Sweden and comparison with previously characterised human strains
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(English)Manuscript (preprint) (Other academic)
National Category
Medical Biotechnology
Research subject
Biomedicine
Identifiers
urn:nbn:se:oru:diva-26575 (URN)
Available from: 2012-12-03 Created: 2012-12-03 Last updated: 2017-10-17Bibliographically approved

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