oru.sePublications
Change search
CiteExportLink to record
Permanent link

Direct link
Cite
Citation style
  • apa
  • harvard1
  • ieee
  • modern-language-association-8th-edition
  • vancouver
  • Other style
More styles
Language
  • de-DE
  • en-GB
  • en-US
  • fi-FI
  • nn-NO
  • nn-NB
  • sv-SE
  • Other locale
More languages
Output format
  • html
  • text
  • asciidoc
  • rtf
Combined real-time PCR and pyrosequencing strategy for objective, sensitive, specific, and high throughput identification of reduced susceptibility to penicillins in Neisseria meningitidis
Örebro University, School of Health and Medical Sciences.
Örebro University, School of Health and Medical Sciences.
Örebro University, Department of Health Sciences.
2008 (English)In: Antimicrobial Agents and Chemotherapy, ISSN 0066-4804, E-ISSN 1098-6596, Vol. 52, no 2, p. 753-756Article in journal (Refereed) Published
Abstract [en]

A segment of penA in Neisseria meningitidis strains (n = 127), including two nucleotide sites closely associated to reduced susceptibility to penicillins, was amplified and pyrosequenced. All results were in concordance with Sanger sequencing, and a high correlation between alterations in the two Pen(i)-specific sites and reduced susceptibility to penicillins was identified.

Place, publisher, year, edition, pages
Washington, DC: American Society for Microbiology , 2008. Vol. 52, no 2, p. 753-756
Keywords [en]
Anti-Bacterial Agents/pharmacology, Bacterial Proteins/chemistry/genetics, Base Sequence, Humans, Microbial Sensitivity Tests/methods, Molecular Sequence Data, Neisseria meningitidis/*drug effects, Penicillin Resistance, Penicillin-Binding Proteins/chemistry/genetics, Penicillins/*pharmacology, Polymerase Chain Reaction/methods, Sensitivity and Specificity, Sequence Analysis; DNA
National Category
Medical and Health Sciences Infectious Medicine
Research subject
Infectious Diseases; Medicine
Identifiers
URN: urn:nbn:se:oru:diva-3451DOI: 10.1128/AAC.00914-07PubMedID: 18070955OAI: oai:DiVA.org:oru-3451DiVA, id: diva2:137748
Available from: 2008-12-08 Created: 2008-12-08 Last updated: 2017-12-14Bibliographically approved
In thesis
1. Antibiotic susceptibility and resistance in Neisseria meningitidis: phenotypic and genotypic characteristics
Open this publication in new window or tab >>Antibiotic susceptibility and resistance in Neisseria meningitidis: phenotypic and genotypic characteristics
2009 (English)Doctoral thesis, comprehensive summary (Other academic)
Abstract [en]

Neisseria meningitidis, also known as the meningococcus, is a globally spread obligate human bacterium causing meningitis and/or septicaemia. It is responsible for epidemics in both developed and developing countries. Untreated invasive meningococcal disease is often fatal, and despite modern intensive care units, the mortality is still remarkably high (approximately 10%). The continuously increasing antibiotic resistance in many bacterial pathogens is a serious public health threat worldwide and there have been numerous reports of emerging resistance in meningococci during the past decades.

In paper I, the gene linked to reduced susceptibility to penicillins, the penA gene, was examined. The totally reported variation in all published penA genes was described. The penA gene was highly variable (in total 130 variants were identified). By examination of clinical meningococcal isolates, the association between penA gene sequences and penicillin susceptibility could be determined. Isolates with reduced susceptibility displayed mosaic structures in the penA gene. Two closely positioned nucleotide polymorphisms were identified in all isolates with reduced penicillin susceptibility and mosaic structured penA genes. These alterations were absent in all susceptible isolates and were successfully used to detect reduced penicillin susceptibility by real-time PCR and pyrosequencing in paper II. In papers III and IV, antibiotic susceptibility and characteristics of Swedish and African meningitis belt meningococcal isolates were comprehensively described. Although both populations were mainly susceptible to the antibiotics used for treatment and prophylaxis, the proportion of meningococci with reduced penicillin susceptibility was slightly higher in Sweden. A large proportion of the African isolates was resistant to tetracycline and erythromycin. In paper V, the gene linked to rifampicin resistance, the rpoB gene, was examined in meningococci from 12 mainly European countries. Alterations of three amino acids in the RpoB protein were found to always and directly lead to rifampicin resistance. A new breakpoint for rifampicin resistance in meningococci was suggested. The biological cost of the RpoB alterations was investigated in mice. The pathogenicity/virulence was significantly lower in rifampicin resistant mutants as compared with susceptible wild-type bacteria.

Place, publisher, year, edition, pages
Örebro: Örebro universitet, 2009. p. 94
Series
Örebro Studies in Medicine, ISSN 1652-4063 ; 38
Keywords
Neisseria meningitidis, meningococcal disease, antibiotic resistance, antibiotic susceptbility, biological cost, PCR, sequencing
National Category
Cell and Molecular Biology Microbiology in the medical area Microbiology in the medical area Microbiology in the medical area
Research subject
Biomedicine; Medicine
Identifiers
urn:nbn:se:oru:diva-8652 (URN)978-91-7668-702-4 (ISBN)
Public defence
2009-12-18, Wilandersalen, Universitetssjukhuset Örebro, Örebro, 09:00 (English)
Opponent
Supervisors
Available from: 2009-11-25 Created: 2009-11-25 Last updated: 2018-01-12Bibliographically approved

Open Access in DiVA

No full text in DiVA

Other links

Publisher's full textPubMedhttp://www.ncbi.nlm.nih.gov/entrez/query.fcgi?db=PubMed&cmd=Retrieve&list_uids=18070955&dopt=Citation

Authority records BETA

Hedberg, Sara ThulinFredlund, HansUnemo, Magnus

Search in DiVA

By author/editor
Hedberg, Sara ThulinFredlund, HansUnemo, Magnus
By organisation
School of Health and Medical SciencesDepartment of Health Sciences
In the same journal
Antimicrobial Agents and Chemotherapy
Medical and Health SciencesInfectious Medicine

Search outside of DiVA

GoogleGoogle Scholar

doi
pubmed
urn-nbn

Altmetric score

doi
pubmed
urn-nbn
Total: 81 hits
CiteExportLink to record
Permanent link

Direct link
Cite
Citation style
  • apa
  • harvard1
  • ieee
  • modern-language-association-8th-edition
  • vancouver
  • Other style
More styles
Language
  • de-DE
  • en-GB
  • en-US
  • fi-FI
  • nn-NO
  • nn-NB
  • sv-SE
  • Other locale
More languages
Output format
  • html
  • text
  • asciidoc
  • rtf