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Comparison of microscopy, culture and in-house PCR and NASBA assays for diagnosis of Neisseria gonorrhoeae in Russia
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2008 (English)In: Acta Pathologica, Microbiologica et Immunologica Scandinavica (APMIS), ISSN 0903-4641, E-ISSN 1600-0463, Vol. 116, no 2, p. 133-138Article in journal (Refereed) Published
Abstract [en]

This study aimed to assess the laboratory diagnosis of Neisseria gonorrhoeae in St. Petersburg, Russia. In total, 334 consecutive symptomatic patients were enrolled. Cervical and urethral specimens from women (nΩ286) and urethral specimens from men (nΩ48) were analyzed by microscopy, culture and two in-house NAATs, i.e. polymerase chain reaction (PCR) and nucleic acid sequence-based amplification (NASBA), developed in Russia. All N. gonorrhoeae-positive samples were confirmed using porA pseudogene and 16S rRNA gene sequencing. All methods displayed 100% specificity, i.e. positive predictive values of 100%. Compared to the PCR (most sensitive method in the present study), in women the sensitivity of both microscopy and culture was 31.8%, and that of NASBA was 90.9%. In men, microscopy, culture and NASBA displayed a sensitivity of 75%, 50% and 100%, respectively. The negative predictive values of microscopy, culture, and NASBA were 97.3%, 97.3%, and 99.6% in women, and 97.8%, 95.7%, and 100% in men, respectively. According to the PCR, the prevalences of N. gonorrhoeae were 4.5% (women) and 8.3% (men). In conclusion, both the investigated Russian NAATs displayed a high sensitivity and specificity. However, in general the diagnosis of gonorrhoea in Russia is suboptimal and crucially requires validation, improvements and quality assurance.

Place, publisher, year, edition, pages
Oxford: Blackwell , 2008. Vol. 116, no 2, p. 133-138
Keyword [en]
DNA; Bacterial/chemistry/genetics, Female, Gonorrhea/diagnosis/epidemiology/*microbiology, Humans, Incidence, Male, Neisseria gonorrhoeae/genetics/*isolation & purification, Polymerase Chain Reaction, Predictive Value of Tests, RNA; Ribosomal; 16S/chemistry/genetics, Russia/epidemiology, Self-Sustained Sequence Replication, Sensitivity and Specificity, Urethral Diseases/diagnosis/epidemiology/*microbiology, Uterine Cervical Diseases/diagnosis/epidemiology/*microbiology
National Category
Medical and Health Sciences Infectious Medicine
Research subject
Infectious Diseases
Identifiers
URN: urn:nbn:se:oru:diva-3463PubMedID: 18321364OAI: oai:DiVA.org:oru-3463DiVA, id: diva2:137760
Available from: 2008-12-08 Created: 2008-12-08 Last updated: 2017-12-14Bibliographically approved

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Unemo, Magnus

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