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Molecular analysis of the gut microbiota of identical twins with Crohn's disease
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2008 (English)In: The ISME journal, ISSN 1751-7370, Vol. 2, no 7, p. 716-727Article in journal (Refereed) Published
Abstract [en]

Increasing evidence suggests that a combination of host genetics and the composition of the gut microbiota are important for development of Crohn's disease (CD). Our aim was to study identical twins with CD to determine microbial factors independent of host genetics. Fecal samples were studied from 10 monozygotic twin pairs with CD (discordant n=6 and concordant n=4) and 8 healthy twin pairs. DNA was extracted, 16S rRNA genes were PCR amplified and T-RFLP fingerprints generated using general bacterial and Bacteroides group-specific primers. The microbial communities were also profiled based on their percentage G+C contents. Bacteroides 16S rRNA genes were cloned and sequenced from a subset of the samples. The bacterial diversity in each sample and similarity indices between samples were estimated based on the T-RFLP data using a combination of statistical approaches. Healthy individuals had a significantly higher bacterial diversity compared to individuals with CD. The fecal microbial communities were more similar between healthy twins than between twins with CD, especially when these were discordant for the disease. The microbial community profiles of individuals with ileal CD were significantly different from healthy individuals and those with colonic CD. Also, CD individuals had a lower relative abundance of B. uniformis and higher relative abundances of B. ovatus and B. vulgatus. Our results suggest that genetics and/or environmental exposure during childhood, in part, determine the gut microbial composition. However, CD is associated with dramatic changes in the gut microbiota and this was particularly evident for individuals with ileal CD.

Place, publisher, year, edition, pages
2008. Vol. 2, no 7, p. 716-727
Keywords [en]
Adolescent, Adult, Aged, Bacteroides/classification/genetics/*isolation & purification, Base Composition, Biodiversity, Cohort Studies, Crohn Disease/*microbiology, DNA; Bacterial/genetics, DNA; Ribosomal/genetics, Diseases in Twins/*microbiology, Feces/microbiology, Female, Gastrointestinal Tract/*microbiology, Humans, Male, Middle Aged, Multivariate Analysis, Polymorphism; Restriction Fragment Length, RNA; Ribosomal; 16S/genetics, Sweden
National Category
Medical and Health Sciences Clinical Medicine Gastroenterology and Hepatology
Research subject
Internal Medicine
Identifiers
URN: urn:nbn:se:oru:diva-3529DOI: 10.1038/ismej.2008.37PubMedID: 18401439OAI: oai:DiVA.org:oru-3529DiVA, id: diva2:137826
Available from: 2008-12-08 Created: 2008-12-08 Last updated: 2017-10-18Bibliographically approved

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Halfvarson, JonasTysk, Curt

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