oru.sePublications
Change search
CiteExportLink to record
Permanent link

Direct link
Cite
Citation style
  • apa
  • harvard1
  • ieee
  • modern-language-association-8th-edition
  • vancouver
  • Other style
More styles
Language
  • de-DE
  • en-GB
  • en-US
  • fi-FI
  • nn-NO
  • nn-NB
  • sv-SE
  • Other locale
More languages
Output format
  • html
  • text
  • asciidoc
  • rtf
A new ELISA for the three-spined stickleback (Gasterosteus aculeatus L.) spiggin, using antibodies against synthetic peptide
Örebro University, School of Science and Technology.ORCID iD: 0000-0001-7336-6335
Show others and affiliations
2008 (English)In: Comparative Biochemistry and Physiology - Part C: Toxicology & Pharmacology, ISSN 1532-0456, E-ISSN 1878-1659, Vol. 147, no 1, p. 129-137Article in journal (Refereed) Published
Abstract [en]

The aim of this study was to develop an enzyme-linked immunosorbent (ELISA) assay to quantify spiggin in the three-spined stickleback. Spiggin is a glue protein produced in the kidney of male three-spined stickleback under the control of androgens during the breeding period. Disturbances of spiggin production in male fish and abnormal induction of spiggin in female fish are considered as valuable biomarkers of exposure to (anti-)androgenic chemicals. Polyclonal antibodies against a peptide sequence of spiggin (HRD-16) were used and the specificity of the antibodies was verified by Western blotting and direct ELISA experiments. By using HRD-16 antibodies and spiggin standard preparation, a competitive ELISA was set-up and validated. This assay appears sensitive, with a detection limit of 0.5 U/mL, and specific, as shown by the competition curves, obtained by serial dilution of male and female kidney homogenates, that were parallel to the spiggin standard curves. The ability of the spiggin ELISA to quantify spiggin induction was achieved by exposing male and female three-spined sticklebacks to 0.1 and 1 microg/L of methyltestosterone. The results show a significant dose-dependent induction of spiggin in methyltestosterone-exposed female fish compared to controls.

Place, publisher, year, edition, pages
New York, NY: Elsevier , 2008. Vol. 147, no 1, p. 129-137
Keywords [en]
Androgens/toxicity, Animals, Antibodies, Antibody Specificity, Biological Markers/metabolism, Blotting; Western, Dose-Response Relationship; Drug, Environmental Monitoring/*methods, Enzyme-Linked Immunosorbent Assay, Female, Fish Proteins/immunology/*metabolism, Kidney/drug effects/*metabolism, Male, Methyltestosterone/toxicity, Peptides/*immunology, Reproducibility of Results, Smegmamorpha/*metabolism, Up-Regulation
National Category
Natural Sciences Chemical Sciences Biochemistry and Molecular Biology
Research subject
Biochemistry
Identifiers
URN: urn:nbn:se:oru:diva-3558DOI: 10.1016/j.cbpc.2007.08.007PubMedID: 17921071OAI: oai:DiVA.org:oru-3558DiVA, id: diva2:137856
Available from: 2008-12-09 Created: 2008-12-09 Last updated: 2017-12-14Bibliographically approved

Open Access in DiVA

No full text in DiVA

Other links

Publisher's full textPubMedhttp://www.ncbi.nlm.nih.gov/entrez/query.fcgi?db=PubMed&cmd=Retrieve&list_uids=17921071&dopt=Citation

Authority records BETA

Olsson, Per-Erik

Search in DiVA

By author/editor
Olsson, Per-Erik
By organisation
School of Science and Technology
In the same journal
Comparative Biochemistry and Physiology - Part C: Toxicology & Pharmacology
Natural SciencesChemical SciencesBiochemistry and Molecular Biology

Search outside of DiVA

GoogleGoogle Scholar

doi
pubmed
urn-nbn

Altmetric score

doi
pubmed
urn-nbn
Total: 413 hits
CiteExportLink to record
Permanent link

Direct link
Cite
Citation style
  • apa
  • harvard1
  • ieee
  • modern-language-association-8th-edition
  • vancouver
  • Other style
More styles
Language
  • de-DE
  • en-GB
  • en-US
  • fi-FI
  • nn-NO
  • nn-NB
  • sv-SE
  • Other locale
More languages
Output format
  • html
  • text
  • asciidoc
  • rtf