oru.sePublications
Change search
CiteExportLink to record
Permanent link

Direct link
Cite
Citation style
  • apa
  • ieee
  • modern-language-association-8th-edition
  • vancouver
  • Other style
More styles
Language
  • de-DE
  • en-GB
  • en-US
  • fi-FI
  • nn-NO
  • nn-NB
  • sv-SE
  • Other locale
More languages
Output format
  • html
  • text
  • asciidoc
  • rtf
P53 mediated regulation of metallothionein transcription in breast cancer cells
Örebro University, School of Science and Technology.ORCID iD: 0000-0001-7336-6335
2007 (English)In: Journal of Cellular Biochemistry, ISSN 0730-2312, E-ISSN 1097-4644, Vol. 102, no 6, p. 1571-1583Article in journal (Refereed) Published
Abstract [en]

Recent studies have shown that only breast cancer epithelial cells with intact p53 can induce metallothionein (MT) synthesis after exposure to metals. In this study, the potential role of p53 on regulation of MT was investigated. Results demonstrate that zinc and copper increased metal response elements (MREs) activity and MTF-1 expression in p53 positive MN1 and parental MCF7 cells. However, inactivation of p53 by treatment with pifithrin- or the presence of inactive p53 inhibited MRE-dependent reporter gene expression in response to metals. MTF-1 levels remained unchanged after treatment with zinc in cells with nonfunctional p53. The introduction of wild-type p53 in MDD2 cells, containing nonfunctional p53, enhanced the ability of zinc to increase MRE-dependent reporter gene expression. The cellular level of p21Cip1/WAF1 was increased in MDD2 cells after p53 transfection, confirming the presence of active p53. The treatment of MN1 and parental MCF7 with trichostatin A led to a sixfold increase in the MRE activity in response to zinc. On the contrary, MRE activity remained unaltered in MDD2 cells with inactive p53. The above results demonstrate that activation of p53 is an important factor in metal regulation of MT. J. Cell. Biochem. 102: 1571-1583, 2007.

Place, publisher, year, edition, pages
Wiley-Liss , 2007. Vol. 102, no 6, p. 1571-1583
Keyword [en]
Benzimidazoles/metabolism, Breast Neoplasms/*metabolism/pathology, Carbocyanines/metabolism, Cations; Divalent/pharmacology, Cell Line; Tumor, Copper/pharmacology, DNA-Binding Proteins/genetics/metabolism, Female, Fluorescent Dyes/metabolism, Gene Expression Regulation; Neoplastic, Genes; Reporter, Humans, Luciferases/metabolism, Membrane Potentials/physiology, Metallothionein/*genetics, Metals; Heavy/pharmacology, Mitochondria/physiology, Plasmids, Response Elements/genetics, Time Factors, Transcription Factors/genetics/metabolism, Transcription; Genetic, Transfection, Tumor Suppressor Protein p53/*metabolism, Zinc/pharmacology
National Category
Natural Sciences Chemical Sciences Biochemistry and Molecular Biology
Research subject
Biochemistry
Identifiers
URN: urn:nbn:se:oru:diva-3560DOI: 10.1002/jcb.21381PubMedID: 17477370OAI: oai:DiVA.org:oru-3560DiVA, id: diva2:137858
Available from: 2008-12-09 Created: 2008-12-09 Last updated: 2017-12-14Bibliographically approved

Open Access in DiVA

No full text in DiVA

Other links

Publisher's full textPubMedhttp://www.ncbi.nlm.nih.gov/entrez/query.fcgi?db=PubMed&cmd=Retrieve&list_uids=17477370&dopt=Citation

Authority records BETA

Olsson, Per-Erik

Search in DiVA

By author/editor
Olsson, Per-Erik
By organisation
School of Science and Technology
In the same journal
Journal of Cellular Biochemistry
Natural SciencesChemical SciencesBiochemistry and Molecular Biology

Search outside of DiVA

GoogleGoogle Scholar

doi
pubmed
urn-nbn

Altmetric score

doi
pubmed
urn-nbn
Total: 290 hits
CiteExportLink to record
Permanent link

Direct link
Cite
Citation style
  • apa
  • ieee
  • modern-language-association-8th-edition
  • vancouver
  • Other style
More styles
Language
  • de-DE
  • en-GB
  • en-US
  • fi-FI
  • nn-NO
  • nn-NB
  • sv-SE
  • Other locale
More languages
Output format
  • html
  • text
  • asciidoc
  • rtf