oru.sePublications
Change search
CiteExportLink to record
Permanent link

Direct link
Cite
Citation style
  • apa
  • harvard1
  • ieee
  • modern-language-association-8th-edition
  • vancouver
  • Other style
More styles
Language
  • de-DE
  • en-GB
  • en-US
  • fi-FI
  • nn-NO
  • nn-NB
  • sv-SE
  • Other locale
More languages
Output format
  • html
  • text
  • asciidoc
  • rtf
A Pisum sativum glyoxysomal malate dehydrogenase induced by cadmium exposure
Örebro University, Department of Natural Sciences. (Molekylär biokemi)
Örebro University, Department of Natural Sciences. (Molekylär biokemi)ORCID iD: 0000-0003-3315-8835
2004 (English)In: DNA Sequence, ISSN 1042-5179, E-ISSN 1029-2365, Vol. 15, no 3, p. 206-208Article in journal (Refereed) Published
Abstract [en]

The glyoxysomal malate dehydrogenase (gMDH) catalyses the formation of oxaloacetate from malate during beta-oxidation of fatty acids in the glyoxysome. A partial Pisum sativum L. (cv. Greenfeast) cDNA was first isolated from a suppression subtractive hybridisation cDNA library obtained from heavy metal stressed plants. The full length cDNA was then isolated by rapid amplification of cDNA ends. The translated sequence showed strong similarity to Cucumis sativus and Citrullus lanatus gMDH including a typical glyoxysome-targeting presequence comprising the PTS2 motif and a cleavage site for a cystein-directed protease. Exposure of pea plants to Cd2+ induced expression of the gMDH gene in mature pea leaves indicating that the enzyme is under environmental control in addition to the normal developmental regulation pattern.

Place, publisher, year, edition, pages
2004. Vol. 15, no 3, p. 206-208
Keywords [en]
Amino Acid Sequence, Base Sequence, Blotting; Northern, Cadmium/*toxicity, DNA Primers, DNA; Complementary/genetics, Enzyme Induction/drug effects, Glyoxysomes/*enzymology, Malate Dehydrogenase/*biosynthesis/*genetics, Molecular Sequence Data, Nucleic Acid Amplification Techniques, Peas/enzymology/*genetics, Sequence Analysis; DNA, Sequence Homology
National Category
Medical Biotechnology (with a focus on Cell Biology (including Stem Cell Biology), Molecular Biology, Microbiology, Biochemistry or Biopharmacy)
Research subject
Biochemistry
Identifiers
URN: urn:nbn:se:oru:diva-4140PubMedID: 15497444OAI: oai:DiVA.org:oru-4140DiVA, id: diva2:138439
Available from: 2007-11-06 Created: 2007-11-06 Last updated: 2017-12-14Bibliographically approved

Open Access in DiVA

No full text in DiVA

Other links

PubMedhttp://www.ncbi.nlm.nih.gov/entrez/query.fcgi?db=PubMed&cmd=Retrieve&list_uids=15497444&dopt=Citation

Authority records BETA

Sävenstrand, HelenaStrid, Åke

Search in DiVA

By author/editor
Sävenstrand, HelenaStrid, Åke
By organisation
Department of Natural Sciences
In the same journal
DNA Sequence
Medical Biotechnology (with a focus on Cell Biology (including Stem Cell Biology), Molecular Biology, Microbiology, Biochemistry or Biopharmacy)

Search outside of DiVA

GoogleGoogle Scholar

pubmed
urn-nbn

Altmetric score

pubmed
urn-nbn
Total: 461 hits
CiteExportLink to record
Permanent link

Direct link
Cite
Citation style
  • apa
  • harvard1
  • ieee
  • modern-language-association-8th-edition
  • vancouver
  • Other style
More styles
Language
  • de-DE
  • en-GB
  • en-US
  • fi-FI
  • nn-NO
  • nn-NB
  • sv-SE
  • Other locale
More languages
Output format
  • html
  • text
  • asciidoc
  • rtf