oru.sePublications
Change search
CiteExportLink to record
Permanent link

Direct link
Cite
Citation style
  • apa
  • harvard1
  • ieee
  • modern-language-association-8th-edition
  • vancouver
  • Other style
More styles
Language
  • de-DE
  • en-GB
  • en-US
  • fi-FI
  • nn-NO
  • nn-NB
  • sv-SE
  • Other locale
More languages
Output format
  • html
  • text
  • asciidoc
  • rtf
Six genes strongly regulated by mercury in Pisum sativum roots
Örebro University, Department of Natural Sciences. (Molekylär biokemi)
Örebro University, Department of Natural Sciences. (Molekylär biokemi)ORCID iD: 0000-0003-3315-8835
2004 (English)In: Plant physiology and biochemistry (Paris), ISSN 0981-9428, E-ISSN 1873-2690, Vol. 42, no 2, p. 135-142Article in journal (Refereed) Published
Abstract [en]

Suppression subtractive hybridisation was used to isolate heavy metal-induced genes from Pisum sativum roots hydroponically exposed to 5 microM HgCl2 and 10 microM EDTA. Six genes were induced out of which one, PsHMIP6B, was novel. The other genes (PsSAMT, PsI2'H, PsNDA, PsAPSR, PsPOD) had not previously been isolated from pea and sequenced. All six genes were also induced after exposure to 5 microM HgCl2 in the absence of EDTA. The induction pattern was in some cases different for the two Hg species, demonstrating a quicker response to-free Hg2+ than Hg-EDTA. The stress-specificity of the gene regulation was investigated by hydroponically adding 5 microM Cd2+. Most Hg-induced cDNAs were also induced by Cd2+ but to a smaller extent than after Hg exposure. In addition, the gene expression was also probed for tissue specificity, which showed that all six genes were expressed in roots and not in leaves.

Place, publisher, year, edition, pages
2004. Vol. 42, no 2, p. 135-142
Keywords [en]
Amino Acid Sequence, Cloning; Molecular, Edetic Acid/pharmacology, Gene Expression Regulation; Plant/*drug effects/genetics, Mercury/*pharmacology, Molecular Sequence Data, Peas/drug effects/*genetics, Plant Proteins/chemistry/*genetics, Plant Roots/drug effects/*genetics, Sequence Alignment, Sequence Homology; Amino Acid
National Category
Biochemistry and Molecular Biology
Research subject
Biochemistry
Identifiers
URN: urn:nbn:se:oru:diva-4141DOI: 10.1016/j.plaphy.2003.11.005PubMedID: 15283129OAI: oai:DiVA.org:oru-4141DiVA, id: diva2:138440
Available from: 2007-11-06 Created: 2007-11-06 Last updated: 2017-12-14Bibliographically approved

Open Access in DiVA

No full text in DiVA

Other links

Publisher's full textPubMedhttp://www.ncbi.nlm.nih.gov/entrez/query.fcgi?db=PubMed&cmd=Retrieve&list_uids=15283129&dopt=Citation

Authority records BETA

Sävenstrand, HelenaStrid, Åke

Search in DiVA

By author/editor
Sävenstrand, HelenaStrid, Åke
By organisation
Department of Natural Sciences
In the same journal
Plant physiology and biochemistry (Paris)
Biochemistry and Molecular Biology

Search outside of DiVA

GoogleGoogle Scholar

doi
pubmed
urn-nbn

Altmetric score

doi
pubmed
urn-nbn
Total: 428 hits
CiteExportLink to record
Permanent link

Direct link
Cite
Citation style
  • apa
  • harvard1
  • ieee
  • modern-language-association-8th-edition
  • vancouver
  • Other style
More styles
Language
  • de-DE
  • en-GB
  • en-US
  • fi-FI
  • nn-NO
  • nn-NB
  • sv-SE
  • Other locale
More languages
Output format
  • html
  • text
  • asciidoc
  • rtf