oru.sePublications
Change search
CiteExportLink to record
Permanent link

Direct link
Cite
Citation style
  • apa
  • harvard1
  • ieee
  • modern-language-association-8th-edition
  • vancouver
  • Other style
More styles
Language
  • de-DE
  • en-GB
  • en-US
  • fi-FI
  • nn-NO
  • nn-NB
  • sv-SE
  • Other locale
More languages
Output format
  • html
  • text
  • asciidoc
  • rtf
Identification of a novel nuclear factor-binding site in the Pisum sativum sad gene promoters
Örebro University, Department of Natural Sciences. (Molekylär biokemi)ORCID iD: 0000-0003-3315-8835
2002 (English)In: Biochimica et Biophysica Acta, Gene Structure and Expression, ISSN 0167-4781, E-ISSN 1879-2634, Vol. 1574, no 3, p. 231-244Article in journal (Refereed) Published
Abstract [en]

DNA fragments containing the 5' promoter regions of the Pisum sativum sadA and sadC genes were amplified from genomic DNA, cloned and sequenced. These sequences contain a number of conserved cis-acting elements, which are potentially involved in stress-induced transcription of the sad genes. To determine whether any of the identified elements are active in binding nuclear factors in vitro, 11 60-bp overlapping (by 30 bp) DNA probe fragments covering the proximal sadC promoter sequence (360 bp) were used in electrophoretic mobility shift assays with competition. Binding activities were compared in nuclear extracts from control, UV-B-stressed and wounded pea leaves. The pattern of DNA binding was almost identical with all three extracts, with one 30-bp region being the predominant site for factor binding. Using overlapping sub-fragments of this region, the majority of the specific binding could be attributed to the novel 11-bp GC-rich sequence GTGGCGCCCAC. An almost identical sequence is conserved in the sadA promoter. This motif has features in common with a number of recognised cis-elements, which suggests a possible binding site for factors which play a role in regulating sad gene transcription.

Place, publisher, year, edition, pages
2002. Vol. 1574, no 3, p. 231-244
Keywords [en]
Alcohol Dehydrogenase/*genetics, Base Sequence, Binding Sites, CCAAT-Enhancer-Binding Proteins/chemistry, DNA-Binding Proteins, Electrophoretic Mobility Shift Assay, Molecular Sequence Data, NFI Transcription Factors, Peas/*genetics/radiation effects, Plant Proteins/*genetics, Promoter Regions (Genetics), Transcription Factors, Ultraviolet Rays, Y-Box-Binding Protein 1
National Category
Medical Biotechnology (with a focus on Cell Biology (including Stem Cell Biology), Molecular Biology, Microbiology, Biochemistry or Biopharmacy)
Research subject
Biochemistry
Identifiers
URN: urn:nbn:se:oru:diva-4147DOI: 10.1016/S0167-4781(01)00366-9PubMedID: 11997088OAI: oai:DiVA.org:oru-4147DiVA, id: diva2:138446
Available from: 2007-11-06 Created: 2007-11-06 Last updated: 2017-12-14Bibliographically approved

Open Access in DiVA

No full text in DiVA

Other links

Publisher's full textPubMedhttp://www.ncbi.nlm.nih.gov/entrez/query.fcgi?db=PubMed&cmd=Retrieve&list_uids=11997088&dopt=Citation

Authority records BETA

Strid, Åke

Search in DiVA

By author/editor
Strid, Åke
By organisation
Department of Natural Sciences
In the same journal
Biochimica et Biophysica Acta, Gene Structure and Expression
Medical Biotechnology (with a focus on Cell Biology (including Stem Cell Biology), Molecular Biology, Microbiology, Biochemistry or Biopharmacy)

Search outside of DiVA

GoogleGoogle Scholar

doi
pubmed
urn-nbn

Altmetric score

doi
pubmed
urn-nbn
Total: 425 hits
CiteExportLink to record
Permanent link

Direct link
Cite
Citation style
  • apa
  • harvard1
  • ieee
  • modern-language-association-8th-edition
  • vancouver
  • Other style
More styles
Language
  • de-DE
  • en-GB
  • en-US
  • fi-FI
  • nn-NO
  • nn-NB
  • sv-SE
  • Other locale
More languages
Output format
  • html
  • text
  • asciidoc
  • rtf