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Regulation of gene expression by low levels of ultraviolet-B radiation in Pisum sativum: isolation of novel genes by suppression subtractive hybridisation
Örebro University, Department of Natural Sciences. (Molekylär biokemi)
Örebro University, Department of Natural Sciences. (Molekylär biokemi)ORCID iD: 0000-0003-3315-8835
2002 (English)In: Plant and Cell Physiology, ISSN 0032-0781, E-ISSN 1471-9053, Vol. 43, no 4, p. 402-410Article in journal (Refereed) Published
Abstract [en]

Suppression subtractive hybridisation was used to isolate genes differentially regulated by low levels (UV-B(BE,300) 0.13 W m(-2)) of ultraviolet-B radiation (UV-B; 290-320 nm) in Pisum sativum. Six genes were regulated, two of which were novel. The mRNA levels for these two (PsTSDC and PsUOS1) were increased and depressed by UV-B treatment, respectively. Domains in the PsTSDC translation product was similar to TIR (Toll-Interleukin-1 receptor-similar) domains and a NB-ARC domain (nucleotide-binding domain in APAF-1, R gene products and CED-4). The PsUOS1 translation product was similar to an open reading frame in Arabidopsis. Genes encoding embryo-abundant protein (PsEMB) and S-adenosyl-L-methionine synthase (PsSAMS) were induced by UV-B, whereas the transcript levels for genes encoding sucrose transport protein (PsSUT) or ribulose-5-phosphate 3-epimerase (PsR5P3E) were decreased. These regulation patterns are novel, and the PsEMB and PsR5P3E sequences are reported for the first time. The stress-specificity of regulation of these genes were tested by ozone fumigation (100 ppb O(3)). Qualitatively, the similarity of expression after both UV-B and ozone exposure suggests that, for these genes, similar stress-response pathways are in action.

Place, publisher, year, edition, pages
2002. Vol. 43, no 4, p. 402-410
Keywords [en]
Amino Acid Sequence, Arabidopsis/genetics/metabolism/radiation effects, Blotting, Northern, Carbohydrate Epimerases/genetics/metabolism, Cloning, Molecular, Ferredoxin-NADP Reductase/genetics/metabolism, Gene Expression Regulation, Plant/drug effects/*radiation effects, Membrane Transport Proteins/genetics/metabolism, Molecular Sequence Data, Nucleic Acid Hybridization/*methods, Ozone/pharmacology, Peas/*genetics/metabolism/radiation effects, Plant Proteins/*genetics/metabolism/radiation effects, RNA, Messenger/genetics/metabolism, Seeds/genetics/metabolism, Sequence Homology, Amino Acid, Ultraviolet Rays
National Category
Medical Biotechnology (with a focus on Cell Biology (including Stem Cell Biology), Molecular Biology, Microbiology, Biochemistry or Biopharmacy)
Research subject
Biochemistry
Identifiers
URN: urn:nbn:se:oru:diva-4149DOI: 10.1093/pcp/pcf047PubMedID: 11978868OAI: oai:DiVA.org:oru-4149DiVA, id: diva2:138448
Available from: 2007-11-06 Created: 2007-11-06 Last updated: 2017-12-14Bibliographically approved

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Publisher's full textPubMedhttp://www.ncbi.nlm.nih.gov/entrez/query.fcgi?db=PubMed&cmd=Retrieve&list_uids=11978868&dopt=Citation

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Sävenstrand, HelenaStrid, Åke

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